A MONOCLONAL ANTIBODY-BASED IMMUNOPEROXIDASE PROCEDURE FOR RAPID DETECTION OF INFECTIOUS-BRONCHITIS VIRUS IN INFECTED-TISSUES
A MONOCLONAL ANTIBODY-BASED IMMUNOPEROXIDASE PROCEDURE FOR RAPID DETECTION OF INFECTIOUS-BRONCHITIS VIRUS IN INFECTED-TISSUES
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DOI：
10.2307/1591379
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发表时间：
1990-10-01
期刊：
影响因子：
1.4
通讯作者：
NAQI, SA
中科院分区：
文献类型：
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作者：
NAQI, SA
采用基于单克隆抗体（MAb）的间接免疫过氧化物酶（IP）方法检测冷冻组织切片中传染性支气管炎病毒（IBV）抗原。该方法可在接种后15小时内在鸡的绒毛膜尿囊膜（CAMs）中检测到IBV抗原，在接种后96小时内在鸡的呼吸组织中检测到IBV抗原。用周期酸处理鸡组织中内源性过氧化物酶。IP技术具有灵敏度高、方便、经济等优点。它允许同时评估抗原携带细胞和一般组织形态。ip染色的载玻片也提供了永久记录。
A monoclonal antibody (MAb)-based indirect immunoperoxidase (IP) procedure was applied to detect infectious bronchitis virus (IBV) antigen in frozen tissue sections. With this procedure, IBV antigen could be detected in chorioallantoic membranes (CAMs) within 15 hr postinoculation and in the respiratory tissues of chickens within 96 hr postinoculation. Endogenous peroxidases in chicken tissues were removed by treatment with periodic acid. The IP technique was highly sensitive, convenient, and economical. It allowed simultaneous evaluation of antigen-bearing cells and the general tissue morphology. The IP-stained slides also provided a permanent record.