Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene
Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene
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DOI：
10.1093/emboj/19.21.5875
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发表时间：
2000-11-01
期刊：
影响因子：
11.4
通讯作者：
Simpson, RT
中科院分区：
文献类型：
--
作者：
Gavin, IM;Kladde, MP;Simpson, RT
在酵母中，许多仅在特定细胞类型中表达的调节蛋白以组合方式与一般转录因子相互作用以控制细胞类型特异性基因的表达。我们报告了一个详细的分析激活和抑制事件发生在α-细胞特异性STE 6基因的启动子融合到β-半乳糖苷酶基因在酵母微型染色体，以及控制染色质结构的启动子在微型染色体和基因组STE 6基因座。Mcm 1 p导致染色质重塑，并负责野生型α和α细胞中STE 6启动子的所有转录活性。Mat alpha 2 p与Tup 1 p合作阻断染色质重塑和Mcm 1 p相关激活。虽然Mata 2 p只抑制Mcm 1 p，但Tup 1 p介导的抑制涉及Mcm 1 p依赖性和非依赖性机制，完全诱导STE 6基因所需的Swi/Snf和Gcn 5 p对染色质重塑没有贡献。我们认为，Tup 1 p可以通过阻断转录激活因子，除了与转录机制和稳定染色质的相互作用，有助于抑制。
In yeast, a number of regulatory proteins expressed only in specific cell types interact with general transcription factors in a combinatorial manner to control expression of cell-type-specific genes. We report a detailed analysis of activation and repression events that occur at the promoter of the a-cell-specific STE6 gene fused to a beta -galactosidase gene in a yeast minichromosome, as well as factors that control the chromatin structure of this promoter both in the minichromosome and in the genomic STE6 locus. Mcm1p results in chromatin remodeling and is responsible for all transcriptional activity from the STE6 promoter in both wild-type a and alpha cells. Mat alpha 2p cooperates with Tup1p to block both chromatin remodeling and Mcm1p-associated activation. While Mata2p represses only Mcm1p, the Tup1p-mediated repression involves both Mcm1p-dependent and -independent mechanisms, Swi/Snf and Gcn5p, required for full induction of the STE6 gene, do not contribute to chromatin remodeling. We suggest that Tup1p can contribute to repression by blocking transcriptional activators, in addition to interacting with transcription machinery and stabilizing chromatin.