ESTABLISHMENT OF ASSAY KITS FOR THE DETERMINATION OF MICROHETEROGENEITIES OF ALPHA-FETOPROTEIN USING LECTIN-AFFINITY ELECTROPHORESIS
ESTABLISHMENT OF ASSAY KITS FOR THE DETERMINATION OF MICROHETEROGENEITIES OF ALPHA-FETOPROTEIN USING LECTIN-AFFINITY ELECTROPHORESIS
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DOI：
10.1016/0009-8981(93)90297-h
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发表时间：
1993-01-31
影响因子：
5
通讯作者：
TAKETA, K
中科院分区：
文献类型：
--
作者：
SHIMIZU, K;TANIICHI, T;TAKETA, K
应用透镜凝集素-A（LCA-A）和红细胞凝集素-植物血凝素-E4（PHA-E4）的凝集素亲和电泳技术，研制了甲胎蛋白（AFP）糖链微异质性诊断试剂盒。用抗体亲和印迹法和免疫酶扩增法对电泳分离的AFP条带进行高灵敏度检测。光密度测定法用于分配凝集素反应性AFP。运行内S.D. AFP条带比例低于3%。在2 ~ 200 ng/ml范围内，条带强度与AFP浓度呈线性关系。比较了55例肝病患者血清中凝集素反应性AFP的分布。慢性肝炎和肝硬化患者凝集素反应性AFP的平均值均低于13%，而肝细胞癌患者凝集素反应性AFP的平均值高于25%。数据与疾病状态的相关性表明，该方法可以极大地促进良恶性肝病的区分。
Diagnostic kits for determination of alpha-fetoprotein (AFP) carbohydrate chain microheterogeneity were developed using lectin affinity electrophoresis with Lens culinaris agglutinin-A (LCA-A) and erythro-agglutinating phytohemagglutinin-E4 (PHA-E4). Separated AFP bands by electrophoresis were detected with high sensitivity by antibody-affinity blotting and immunoenzymatic amplification. Densitometry was used to apportion lectin reactive AFPs. The within-run S.D. for proportions of AFP bands was below 3%. Band intensity was linearly related to AFP concentration between 2 and 200 ng/ml. Profiles of lectin reactive AFPs were compared in serum samples from 55 patients having liver diseases. The average values of lectin reactive AFPs for chronic hepatitis and liver cirrhosis patients were both below 13%, but those of hepatocellular carcinoma patients were above 25%. Correlation of data with disease states suggests that the methods can greatly facilitate the discrimination between benign and malignant liver diseases.