DEVELOPMENT OF SSR MARKERS FROM CITRUS CLEMENTINA (RUTACEAE) BAC END SEQUENCES AND INTERSPECIFIC TRANSFERABILITY IN CITRUS
DEVELOPMENT OF SSR MARKERS FROM CITRUS CLEMENTINA (RUTACEAE) BAC END SEQUENCES AND INTERSPECIFIC TRANSFERABILITY IN CITRUS
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DOI：
10.3732/ajb.1000280
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发表时间：
2010-11-01
影响因子：
3
通讯作者：
Ollitrault, Patrick
中科院分区：
文献类型：
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作者：
Ollitrault, Frederique;Terol, Javier;Ollitrault, Patrick
本研究的目的：方法和结果：采用聚丙烯酰胺凝胶电泳和DNA银染色技术，从北京谱仪（BES）中提取的526个微卫星位点中筛选出79对引物，对引物的多态性和可转移性进行了研究。对柑桔属18个种的多样性进行了初步研究。reticulata、网叶C. maxima，C. medica，C. sinensis、中华绒螯蟹C. aurantium，C. paradisi，C. lemon，C. aurantifolia和一些papedas（野生柑橘），使用毛细管电泳片段分析仪。观察到种内和种间多态性，并确定了不同的基因型用于遗传mapping.Conclusions杂合标记：这些结果表明开发的引物比较映射研究和物理和遗传图谱的整合的效用。
Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping.Methods and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping.Conclusions: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.