Antibodies to the evolutionarily conserved amino-terminal region of the v-myb-encoded protein detect the c-myb protein in widely divergent metazoan species.
Antibodies to the evolutionarily conserved amino-terminal region of the v-myb-encoded protein detect the c-myb protein in widely divergent metazoan species.
复制标题
针对 v-myb 编码蛋白进化上保守的氨基末端区域的抗体可检测差异很大的后生动物物种中的 c-myb 蛋白。
DOI：
10.1073/pnas.83.13.4685
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发表时间：
1986
影响因子：
11.1
通讯作者：
Baluda,MA
中科院分区：
文献类型：
--
作者：
Boyle,WJ;Lipsick,JS;Baluda,MA
针对含有高度进化保守的禽成髓细胞病病毒（AMV） v-myb癌基因5' 1 / 3编码区域的细菌融合蛋白的抗体检测myb基因家族各种成员的蛋白产物。用这些抗体进行免疫沉淀或免疫印迹分析得到以下信息。首先，AMV的v-myb致癌基因（p48v-myb）和E26病毒（p135gag-myb-ets）的产物含有这种高度保守的氨基酸序列，这与之前的假设一致。其次，鸡c-myb原癌基因的产物p75c-myb也含有该蛋白结构域。第三，这些抗体已经确定了人类、小鼠和果蝇c-myb基因的产物，这些基因都被发现是Mr为75,000-80,000的核蛋白。人c-myb蛋白产物存在于红系、髓系和淋巴系的未成熟细胞中。
Antibodies directed against a bacterial fusion protein that contains the domain encoded by the highly evolutionarily conserved 5' one-third of the v-myb oncogene of avian myeloblastosis virus (AMV) detect the protein products of various members of the myb gene family. Immunoprecipitation or immunoblot analyses with these antibodies yielded the following information. First, the products of the v-myb oncogenes of AMV (p48v-myb) and of E26 virus (p135gag-myb-ets) contain this highly conserved amino acid sequence, as previously hypothesized. Second, p75c-myb, the product of the chicken c-myb protooncogene, also contains this protein domain. Third, these antibodies have identified the products of the human, murine, and Drosophila c-myb genes, which were all found to be nuclear proteins of Mr 75,000-80,000. The human c-myb protein product is present in immature cells of the erythroid, myeloid, and lymphoid lineages.