The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations.
The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations.
复制标题
小鼠 IL 2 受体。
作者：
Thomas R. Malek;J. Schmidt;E. Shevach
用抗IL 2受体的单抗直接测定凝集素ConA诱导小鼠T细胞亚群产生IL 2受体所需的辅助细胞。在辅助细胞存在和不存在的情况下，MHC I类限制性、抑制性/细胞毒性表型(L3T4-、Ly-2+)的T淋巴细胞均可诱导大量IL 2受体的表达。相反，只有MHC II类限制性辅助者/诱导者表型(L3T4+，LY-2-)的T细胞才能诱导高水平的IL-2受体表达。在为L3T4+，Ly-2-亚群提供辅助细胞功能方面，IA-细胞如P388D-巨噬细胞系或培养的成纤维细胞(DAP X-3)与Ia+B细胞杂交瘤Lb细胞一样有效。PMA可替代辅细胞表达IL-2受体和L3T4+、Ly-2-亚群分泌IL-2，但不能替代纯化的人IL-1。提示IL-2受体在L3T4+，Ly-2-T细胞上的诱导是复杂的，可能需要T细胞与辅助细胞的相互作用，而凝集素可能直接触发L3T4-，Ly-2+T细胞上IL-2受体的表达。
The accessory cell requirements for the induction of the IL 2 receptor by the lectin Con A on murine T cell subsets were directly assayed with anti-IL 2 receptor monoclonal antibodies. Substantial levels of IL 2 receptor expression were induced on T lymphocytes of the MHC class I-restricted, suppressor/cytotoxic phenotype (L3T4-, Ly-2+) in the presence and absence of accessory cells. In contrast, high levels of IL 2 receptor expression could only be induced on T cells of the MHC class II-restricted, helper/inducer phenotype (L3T4+, LY-2-) in the presence, but not in the absence, of accessory cells. Ia- cells such as the P388D1 macrophage line or cultured fibroblasts (DAP X 3) were as efficient as the Ia+ B cell hybridoma LB in providing accessory cell function for the L3T4+, Ly-2- subset. PMA, but not purified human IL 1, could substitute for accessory cells for both IL 2 receptor expression and IL 2 secretion by the L3T4+, Ly-2- subset. These data suggest that IL 2 receptor induction on the L3T4+, Ly-2- subset is complex, possibly requiring a T cell-accessory cell interaction, whereas the lectin may directly trigger IL 2 receptor expression on L3T4-, Ly-2+ T cells.