Detection and Identification of Malaria Parasites: A Review of Proficiency Test Results and Laboratory Practices

Detection and Identification of Malaria Parasites: A Review of Proficiency Test Results and Laboratory Practices
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DOI:
10.1309/lm0kc4beyhgdszcu
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发表时间:
2010-12-01
期刊:
影响因子:
--
通讯作者:
Massey, Laura D.
Massey, Laura D.
中科院分区:
医学4区
文献类型:
--
作者:
Edson, Daniel C.;Glick, Teri;Massey, Laura D.

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背景 本研究评估了检测和识别疟疾寄生虫的能力验证 (PT) 表现,并检查了美国实验室的疟疾筛查实践。方法 我们回顾了 1999 年至 2008 年的 PT 数据,以确定检测和识别疟原虫的不可接受反应的比率。我们还调查了当前 PT 参与者,以评估疟疾筛查实践。疟疾、间日疟原虫和卵形疟原虫 半数接受调查的实验室未能提供完整的诊断检查 25 0% 未能筛查厚玻片和薄玻片,36 1% 未能在 24 小时内发布最终报告 结论 教育计划应侧重于识别疟原虫并区分寄生虫和寄生虫 实验室应在内部或通过转介提供完整的诊断检查,并确保在 24 小时内获得最终结果
Background This study assesses proficiency test (PT) performance in detecting and identifying malaria parasites and examines malaria screening practices in laboratories in the United StatesMethod We reviewed PT data from 1999 to 2008 to determine the rates of unacceptable responses for detecting and identifying Plasmodium sp We also surveyed current PT participants to assess malaria screening practicesResults Laboratories were most proficient at detecting and identifying P falciparum and substantially less proficient at identifying P malariae, P vivax and P ovale Half of the surveyed laboratories failed to offer a complete diagnostic workup 25 0% failed to screen both thick and thin slides, and 36 1% failed to issue a final report within 24 hoursConclusion Educational programs should focus on identifying Plasmodium sp and distinguishing artifacts from parasites Laboratories should offer a complete diagnostic workup, either in house or by referral and ensure final results within 24 hours