HLA-A, -B, -DR haplotype frequencies from DNA typing data of 26,266 Chinese bone marrow donors
HLA-A, -B, -DR haplotype frequencies from DNA typing data of 26,266 Chinese bone marrow donors
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DOI：
10.1016/j.humimm.2007.04.006
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发表时间：
2007-10-01
期刊：
影响因子：
2.7
通讯作者：
Zhao, Tong-Mao
中科院分区：
文献类型：
--
作者：
Du, Ke-Ming;Ji, Yun;Zhao, Tong-Mao
上海红十字会骨髓捐献者登记处（中国骨髓捐献者计划的一部分）招募了 26,266 名中国人作为潜在的造血干细胞捐献者，他们使用基于 DNA 的分型方法以低至中分辨率测定了 HLA-A、-B 和 -DRB1 等位基因。该研究的大样本量使得能够准确计算中国人HLA单倍型频率。观察到的等位基因对应于 19 HLA-A、44 -B 和 13 -DR 裂解抗原。未观察到 HLA-A36、-A80、-B78 和 -DR18 等位基因的血清学等效物。总共鉴定出 2,241 个不同的 HLA-A、-B、-DRB1 单倍型。使用最大似然法估计三基因座单倍型频率。可以在 95% 置信水平下可靠估计的最低单倍型频率为 0.000057。使用此截止值，1,220 个单倍型 (54%) 在统计上是可靠的，其累积单倍型频率为 0.9730。其余 1,021 个单倍型 (46%) 的累积单倍型频率为 0.0270。推导出 p = 0.192 log N-0.576 的回归方程，以估计在 N 个中国捐献者库中找到 HLA-A、-B、-DR 分裂抗原匹配捐献者的概率 (p)。 (c) 2007 年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
HLA phenotypes of 26,266 Chinese individuals who were recruited as potential hematopoietic stem cell donors by the Shanghai Red Cross Marrow Donor Registry, part of the China Marrow Donor Program, were determined for HLA-A, -B, and -DRB1 alleles at low to intermediate resolution using DNA-based typing methods. The large sample size of the study allowed accurate calculation of the Chinese HLA haplotype frequencies. The observed alleles correspond to 19 HLA-A, 44 -B, and 13 -DR split antigens. The serologic equivalents of HLA-A36, -A80, -B78, and -DR18 alleles were not observed. A total of 2,241 distinct HLA-A, -B, -DRB1 haplotypes were identified. Three-locus haplotype frequency was estimated using the maximum likelihood method. The lowest haplotype frequency that can be reliably estimated at a 95% confidence Level was 0.000057. Using this cutoff value, 1,220 haplotypes (54%) were statistically reliable and their cumulative haplotype frequency was 0.9730. The cumulative haplotype frequency of the remaining 1,021 haplotypes (46%) was 0.0270. A regression equation of p = 0.192 log N-0.576 was derived to estimate the probability (p) of finding an HLA-A, -B, -DR split antigens-matched donor in a pool of N Chinese donors. (c) 2007 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.