Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet
Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet
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DOI：
10.1007/s10735-012-9442-y
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发表时间：
2012-08
影响因子：
3.2
通讯作者：
Shigeru Takahashi;Hiroki Uekita;Tsuyoshi Kato;F. Yuge;Natsumi Ushijima;Kiichiro Inoue;T. Domon
中科院分区：
文献类型：
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作者：
Shigeru Takahashi;Hiroki Uekita;Tsuyoshi Kato;F. Yuge;Natsumi Ushijima;Kiichiro Inoue;T. Domon
据报告，喂食流质饲料的实验动物的腮腺出现萎缩（Hall和Schneyer 1964; Wilborn和Schneyer 1970; Hand和Ho 1981; Scott等人1990; Scott和古恩1991）。为了阐明腺泡细胞的凋亡和增殖是否参与流质饮食诱导的大鼠腮腺萎缩，给大鼠喂食流质饮食，并与喂食颗粒的对照组进行比较。在第3、7、14或21天取出腮腺，称重，并使用透射电子显微镜（TEM）进行检查，并对凋亡细胞的标记物切割半胱天冬酶-3（Casp-3）和增殖细胞的标记物5-溴-2 ′-脱氧尿苷（BrdU）进行化学研究。喂流质饲料的实验大鼠体重与喂颗粒饲料的对照组无显着差异，但实验腮腺的重量小于对照组。在实验腮腺中，在每个时间点在腺泡中组织学上观察到凋亡小体等结构;在第3、7和14天，在实验腮腺中鉴定出比对照组更多的Casp-3阳性腺泡细胞。实验腺体在每个时间点显示较少的BrdU阳性腺泡细胞。透射电镜证实萎缩腺体内有典型的腺泡细胞凋亡。这些结果表明，增加腺泡细胞凋亡和减少腺泡细胞增殖发生在萎缩的大鼠腮腺喂流质饮食。
Parotid glands of experimental animals fed a liquid diet are reported to show atrophy (Hall and Schneyer 1964; Wilborn and Schneyer 1970; Hand and Ho 1981; Scott et al. 1990; Scott and Gunn 1991). To clarify whether apoptosis and proliferation of acinar cells participate in atrophy of rat parotid glands induced by liquid diet, rats were fed a liquid diet and compared to pellet-fed controls. Parotid glands were removed at 3, 7, 14 or 21 days, weighed, and examined using transmission electron microscopy (TEM), and studied immunohistochemically for cleaved-caspase-3 (Casp-3), a marker of apoptotic cells, and 5-bromo-2′-deoxyuridine (BrdU), a marker for proliferating cells. Body weights of experimental rats fed liquid diets were not significantly different from controls fed pellet diets; however weights of experimental parotid glands were smaller than those of controls. In the experimental parotid glands, structures like apoptotic bodies were histologically observed in acini at each time point; more Casp-3-positive acinar cells were identified in experimental parotid glands than in the controls on days 3, 7, and 14. Experimental glands showed fewer BrdU-positive acinar cells at each time point. TEM confirmed typical apoptotic acinar cells in the atrophic glands. These findings suggest that increased acinar cell apoptosis and reduced acinar cell proliferation occur in atrophic parotid glands of rats fed a liquid diet.