Comparison of the Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue
Comparison of the Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue
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DOI：
10.1007/s11745-001-0843-0
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发表时间：
2001-11-01
期刊：
影响因子：
1.9
通讯作者：
Cooper, MH
中科院分区：
文献类型：
--
作者：
Iverson, SJ;Lang, SLC;Cooper, MH
对于许多研究来说，准确测量生物样品的总脂质含量非常重要。 Bligh 和 Dyer 提取方法被开发为一种快速而有效的测定鱼肌肉中总脂质含量的方法。然而，它也广泛用于测量全鱼和其他组织的总脂质含量的研究。尽管一些研究人员可能使用了修改后的布莱和戴尔程序，但很少具体说明修改，也没有对其有效性进行定量评估。因此，我们将该方法与经典的 Folch 提取方法在测定鱼类样品总脂质含量（脂质含量为 0.5% 至 26.6%）方面进行了比较。我们按照最初指定的方式执行了这两种方法，即对于 Bligh 和 Dyer 使用 1:2:0.8 和 2:2:1.8（分别在稀释之前和之后）的氯仿/甲醇/水比例，对于 Folch 使用 8:43 的氯仿/甲醇/水比例，并且初始溶剂/样品比例为 (3+1):1 (Bligh 和 Dyer) 和 20:1 (Folch)。我们还将这些与其他几种溶剂/样品比率进行了比较。在含有 2% 脂质的样品中，Bligh 和 Dyer 方法得出的脂质含量估计值明显较低，并且这种低估随着样品脂质含量的增加而显着增加。在最高脂质样品中，使用 Bligh 和 Dyer 方法，脂质含量被低估高达 50%。然而，我们发现这两种方法之间存在高度显着的线性关系，这将允许校正先前使用未经修改的 Bligh 和 Dyer 提取分析的样品中报告的脂质水平。将来，应描述对程序和溶剂/样品比例的修改。
For many studies, it is important to measure the total lipid content of biological samples accurately. The Bligh and Dyer method of extraction was developed as a rapid but effective method for determining total lipid content in fish muscle. However, it is also widely used in studies measuring total lipid content of whole fish and other tissues. Although some investigators may have used modified Bligh and Dyer procedures, rarely have modifications been specified nor has their effectiveness been quantitatively evaluated. Thus, we compared this method with that of the classic Folch extraction in determining total lipid content of fish samples ranging from 0.5 to 26.6% lipid. We performed both methods as originally specified, i.e., using the chloroform/methanol/water ratios of 1:2:0.8 and 2:2:1.8 (before and after dilution, respectively) for Bligh and Dyer and of 8:43 for Folch, and with the initial solvent/sample ratios of (3+1):1 (Bligh and Dyer) and 20:1 (Folch). We also compared these with several other solvent/sample ratios. In samples containing 2% lipid, the Bligh and Dyer method produced significantly lower estimates of lipid content, and this underestimation increased significantly with increasing lipid content of the sample. in the highest lipid samples, lipid content was underestimated by up to 50% using the Bligh and Dyer method. However, we found a highly significant linear relationship between the two methods, which will permit the correction of reported lipid levels in samples previously analyzed using an unmodified Bligh and Dyer extraction. In the future, modifications to procedures and solvent/sample ratios should be described.