Physicochemical analysis of (Pro‐Pro‐Gly)n with defined molecular weight–temperature dependence of molecular weight in aqueous solution
Physicochemical analysis of (Pro‐Pro‐Gly)n with defined molecular weight–temperature dependence of molecular weight in aqueous solution
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水溶液中具有确定分子量的 (Pro-Pro-Gly)n 的物理化学分析 - 分子量的温度依赖性
DOI：
10.1002/bip.1970.360090404
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发表时间：
1970
期刊：
影响因子：
2.9
通讯作者：
T. Isemura
中科院分区：
文献类型：
--
作者：
Yuji Kobayashi;R. Sakai;K. Kakiuchi;T. Isemura
采用固相法合成确定分子量的（Pro‐Pro‐Gly）n, （n = 10,15,20）序列多肽。通过旋光性和沉降平衡的测量，研究了这些样品的构象随温度的变化。旋光性的温度依赖性与胶原蛋白分子的热跃迁相似。在较低的温度下，每一种聚合物都作为计时器存在。（Pro‐Pro‐Gly）10在较高的温度下作为单体存在，而其他化合物的行为也类似。
Sequenced polytripeptides, (Pro‐Pro‐Gly)n, (n = 10, 15, 20), with defined molecular weights were synthesized by the solid‐phase method. Conformational changes of these sample as a function of temperature were studied by measurements of optical rotation and sedimentation equilibrium. The temperature dependence of optical rotation was shown similar to thermal transition of collagen molecule. Each of these polymers existed as a timer at lower temperature. (Pro‐Pro‐Gly)10 existed as a monomer at higher temperature, and the others were expected to behave analogously.