Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica napus
Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica napus
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DOI：
10.1093/jxb/49.323.1031
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发表时间：
1998-06
影响因子：
6.9
通讯作者：
E. Larsson;J. Bornman;H. Asp
中科院分区：
文献类型：
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作者：
E. Larsson;J. Bornman;H. Asp
以甘蓝型油菜（Brassica napus L.）为材料，研究了UV-B辐射和镉胁迫同时处理的可能互作效应。CV. Paroll关于叶绿素荧光，生长和吸收选定的元素。植物生长在含CdCl 2（0、0.5、2或5 μM）的营养液中，用光合有效辐射（PAR，40 0 - 70 0 nm，80 0 μmol m-2 s-1）照射，并辅以或不辅以紫外线B（UV-B，2 80 - 32 0 nm，15 kJ m-2 d-1，加权辐照度）。处理14 d后，在2和5 μM CdCl 2 + UV-B辐射和不加UV-B辐射的条件下，处理效果最明显。暴露于镉显着增加了镉在根和地上部的量。此外，根系中Fe、Zn、Cu和P的含量增加，而K的含量减少。在地上部的S含量上升显着的存在和不存在的UV-B辐射，而Mg，Ca，P，Cu，和K的显着增加只发生在植物暴露于Cd和UV-B辐射。锰在联合暴露处理下显著降低。硫含量的上升可能是由于刺激谷胱甘肽和phytochelatin的合成。镉暴露显著降低了根干重、叶面积、总叶绿素含量、类胡萝卜素含量和光合作用的光化学量子效率。作为光合作用能量耗散过程的一种估计，非光化学猝灭（qNPQ）是用脉冲幅度调制荧光计测量的。q NPQ随Cd的增加而增加，而Cd和UV-B的组合降低了q NPQ相比，仅暴露于镉或UV-B辐射的植物。叶绿素a：B比值在无镉或低镉浓度（0 μM、0.5 μ MCdCl 2）下随UV-B的增加而降低，但在高镉浓度（2 μM、5 μ MCdCl 2）下不降低。因此，在某些情况下，似乎是一个UV-B和镉的相互作用，而在其他植物的反应可以归因于单独的治疗。
The possible interaction of two stresses, UV-B radiation and cadmium, applied simultaneously, was investigated in Brassica napus L. cv. Paroll with respect to chlorophyll fluorescence, growth and uptake of selected elements. Plants were grown in nutrient solution containing CdCl 2 , (0, 0.5, 2 or 5 μM) and irradiated with photosynthetically active radiation (PAR, 400-700 nm, 800 μmol m -2 s -1 ) with or without supplemental ultraviolet-B radiation (UV-B, 280-320 nm, 15 kJ m -2 d -1 , weighted irradiance). After 14 d of treatment, the most pronounced effects were found at 2 and 5 μM CdCl 2 with and without supplemental UV-B radiation. Exposure to cadmium significantly increased the amount of Cd in both roots and shoots. In addition, increases occurred in the concentration of Fe, Zn, Cu, and P in roots, while K was reduced. In shoots the S content rose significantly both in the presence and absence of UV-B radiation, while significant increases in Mg, Ca, P, Cu, and K occurred only in plants exposed to Cd and UV-B radiation. Manganese decreased significantly under the combined exposure treatment. The rise in S content may have been due to stimulated glutathione and phytochelatin synthesis. Cadmium exposure significantly decreased root dry weight, leaf area, total chlorophyll content, carotenoid content, and the photochemical quantum yield of photosynthesis. As an estimation of energy dissipation processes in photosynthesis, non-photochemical quenching (q NPQ ) was measured using a pulse amplitude modulated fluorometer. The q NPQ increased with increasing Cd, while the combination of cadmium and UV-B reduced the q NPQ compared to that in plants exposed only to cadmium or UV-B radiation. The chlorophyll a:b ratio showed a reduction with UV-B at no or low Cd concentrations (0 μM, 0.5 μM CdCl 2 ), but not at the higher Cd concentrations used (2 μM, 5 μM CdCl 2 ). Thus in some instances there appeared to be a UV-B and Cd interaction, while in others plant response could be attributed to either treatment alone.