Random Walk Behavior of Migrating Cortical Interneurons in the Marginal Zone: Time-Lapse Analysis in Flat-Mount Cortex
Random Walk Behavior of Migrating Cortical Interneurons in the Marginal Zone: Time-Lapse Analysis in Flat-Mount Cortex
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DOI：
10.1523/jneurosci.5446-08.2009
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发表时间：
2009-02
期刊：
影响因子：
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通讯作者：
Daisuke H. Tanaka;Mitsutoshi Yanagida;Yan Zhu;S. Mikami;T. Nagasawa;J. Miyazaki;Y. Yanagawa;K. Obata;F. Murakami
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文献类型：
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作者：
Daisuke H. Tanaka;Mitsutoshi Yanagida;Yan Zhu;S. Mikami;T. Nagasawa;J. Miyazaki;Y. Yanagawa;K. Obata;F. Murakami
迁移的神经元被认为是通过检测细胞外环境中的环境线索，沿着刻板的路径从靠近脑室的起始处移动到遥远的区域。在这里，我们报告了一种新的神经元迁移模式，挑战了这一观点。我们对内侧神经节隆起(MGE)来源的皮质中间神经元进行了长期的时间推移成像，这些神经元在平面区的边缘区(MZ)切向迁移。我们发现，它们在迁徙的速度和方向上表现出不同的行为范围。奇怪的是，这些神经元的主要群体以一种不可预测的方式反复改变其迁移方向。迁移的轨迹因神经元不同而不同。单个细胞的迁移是长时间的，有时长达2d。理论分析表明，这些行为可以用随机游走来模拟。此外，MZ细胞从皮质脑室下区迁移到皮质板，在MZ内短暂积聚。这些结果表明，MGE来源的皮质中间神经元一旦到达MZ，就会从引导线索的调节中释放出来，并开始随机行走，这可能有助于它们分散在整个皮质。
Migrating neurons are thought to travel from their origin near the ventricle to distant territories along stereotypical pathways by detecting environmental cues in the extracellular milieu. Here, we report a novel mode of neuronal migration that challenges this view. We performed long-term, time-lapse imaging of medial ganglionic eminence (MGE)-derived cortical interneurons tangentially migrating in the marginal zone (MZ) in flat-mount cortices. We find that they exhibit a diverse range of behaviors in terms of the rate and direction of migration. Curiously, a predominant population of these neurons repeatedly changes its direction of migration in an unpredictable manner. Trajectories of migration vary from one neuron to another. The migration of individual cells lasts for long periods, sometimes up to 2 d. Theoretical analyses reveal that these behaviors can be modeled by a random walk. Furthermore, MZ cells migrate from the cortical subventricular zone to the cortical plate, transiently accumulating in the MZ. These results suggest that MGE-derived cortical interneurons, once arriving at the MZ, are released from regulation by guidance cues and initiate random walk movement, which potentially contributes to their dispersion throughout the cortex.