Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins
Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins
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使用拟南芥肌动蛋白测量拟南芥肌球蛋白的酶活性和运动活性
DOI：
10.1016/j.bbrc.2017.12.071
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
and Ito K.
中科院分区：
文献类型：
--
作者：
Rula S;Suwa T;Kijima ST;Haraguchi T;Wakatsuki S;Sato N;Duan Z;Tominaga M;Uyeda TQP;and Ito K.
在高等植物中有两类肌球蛋白，即XI和VIII。肌球蛋白XI能高速移动肌动蛋白细丝，其酶活性也很高。相比之下，肌球蛋白VIII移动肌动蛋白细丝的速度非常慢，酶活性非常低。由于大多数这些酶和运动活性是用动物骨骼肌α-肌动蛋白测量的，而不是植物肌动蛋白，它们不能准确地反映植物细胞的实际活性。因此，我们用三种拟南芥肌动蛋白亚型(ACT1、ACT2和ACT7)测定了两个拟南芥球蛋白XI亚型(MYA2、XI-B)和一个拟南芥球蛋白III亚型(ATM1)的运动区的酶活性和运动活性。测量的活动与使用肌肉肌动蛋白测量的活动不同。此外，当使用不同的拟南芥肌动蛋白异构体时，拟南芥肌球蛋白表现出不同的酶活性和运动活性。我们的结果表明，植物肌动蛋白应该被用来测量植物肌球蛋白的酶活性和运动活性，并且植物细胞中不同的肌动蛋白亚型可能作为不同的轨道发挥作用，沿着不同的轨道调节每种肌球蛋白亚型的亲和力和速度。
There are two classes of myosin, XI and VIII, in higher plants. Myosin XI moves actin filaments at high speed and its enzyme activity is also very high. In contrast, myosin VIII moves actin filaments very slowly with very low enzyme activity. Because most of these enzymatic and motile activities were measured using animal skeletal muscle α-actin, but not plant actin, they would not accurately reflect the actual activities in plant cells. We thus measured enzymatic and motile activities of the motor domains of twoArabidopsismyosin XI isoforms (MYA2, XI-B), and oneArabidopsismyosin VIII isoform (ATM1), by using threeArabidopsisactin isoforms (ACT1, ACT2, and ACT7). The measured activities were different from those measured by using muscle actin. Moreover,Arabidopsismyosins showed different enzymatic and motile activities when using differentArabidopsisactin isoforms. Our results suggest that plant actin should be used for measuring enzymatic and motile activities of plant myosins and that different actin isoforms in plant cells might function as different tracks along which affinities and velocities of each myosin isoform are modulated.