Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
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DOI：
10.1099/ijs.0.02441-0
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Huber, T
中科院分区：
文献类型：
--
作者：
Hugenholtz, P;Huber, T
通过部分树分析，在公共数据库中鉴定出了大量不同来源的嵌合 16S rDNA 序列。这表明，尽管研究人员普遍认识到 PCR 产生的假象，但代表系统发育上新颖的不存在生物体的嵌合序列在分子系统发育调查中经常被忽视。
A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.