Human unrestricted somatic stem cells loaded in nanofibrous PCL scaffold and their healing effect on skin defects
Human unrestricted somatic stem cells loaded in nanofibrous PCL scaffold and their healing effect on skin defects
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DOI：
10.3109/21691401.2015.1062390
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发表时间：
2016-09-01
影响因子：
5.8
通讯作者：
Biazar, Esmaeil
中科院分区：
文献类型：
--
作者：
Bahrami, Hoda;Keshel, Saeed Heidari;Biazar, Esmaeil
利用纳米纤维聚己内酯（PCL）支架负载不受限制的体细胞干细胞（USSCs），将其移植到大鼠全层皮肤缺损上，可用于皮肤再生。采用静电纺丝法设计了纳米纤维PCL支架，并与层粘连蛋白交联。然后对支架进行扫描电镜和物理力学分析。本研究将负载USSCs的纳米纤维PCL支架移植到皮肤缺损上。移植后21天对伤口进行检查。力学和物理分析结果显示，作为皮肤移植物具有良好的弹性和运动顺应性。在动物模型中；研究样本对伤口愈合的影响最为显著，术后21天伤口愈合有统计学上的显著改善。所有样本愈合伤口的组织学检查显示，有细胞样本的真皮层有薄表皮和恢复的皮肤附属物。由此可见，负载USSC的纳米纤维PCL支架移植在大鼠皮肤缺损愈合过程中表现出较好的效果。
Unrestricted somatic stem cells (USSCs) loaded in nanofibrous polycaprolactone (PCL) scaffolds can be used for skin regeneration when grafted onto full-thickness skin defects of rats. Nanofibrous PCL scaffolds were designed by the electrospinning method and crosslinked with laminin protein. Afterwards, the scaffolds were evaluated by scanning electron microscopy, and physical and mechanical assays. In this study, nanofibrous PCL scaffolds loaded with USSCs were grafted onto the skin defects. The wounds were subsequently investigated 21 days after grafting. Results of mechanical and physical analyses showed good resilience and compliance to movement as a skin graft. In animal models; study samples exhibited the most pronounced effect on wound closure, with statistically significant improvement in wound healing being seen at 21 days post-operatively. Histological examinations of healed wounds from all samples showed a thin epidermis plus recovered skin appendages in the dermal layer for samples with cell. Thus, the graft of nanofibrous PCL scaffolds loaded with USSC showed better results during the healing process of skin defects in rat models.