Catecholamine Clearance from Alveolar Spaces of Rat and Human Lungs
Catecholamine Clearance from Alveolar Spaces of Rat and Human Lungs
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DOI：
10.1159/000084051
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发表时间：
2005-03
期刊：
影响因子：
3.7
通讯作者：
T. Sakuma;X. Gu;M. Sugita;M. Sagawa;M. Sakuda;H. Toga
中科院分区：
文献类型：
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作者：
T. Sakuma;X. Gu;M. Sugita;M. Sagawa;M. Sakuda;H. Toga
工作背景：尽管雾化β-肾上腺素能激动剂已被用作缓解肺水肿的治疗方法，但从肺泡间隙清除儿茶酚胺的机制尚不清楚。目的：探讨肺泡腔内儿茶酚胺清除率与肺液体转运能力的关系。研究方法：将含有肾上腺素（10- 7 M）或去甲肾上腺素（10- 7 M）的白蛋白溶液滴入离体大鼠和人肺的肺泡腔中。通过1小时内白蛋白浓度的逐渐增加来估计肺泡液清除率。通过观察1h内肺泡液中儿茶酚胺浓度和肺泡液量的变化来评价儿茶酚胺清除率。结果：去甲肾上腺素在大鼠和人肺中的清除速率均快于肾上腺素。在大鼠肺中，阿米洛利（一种钠通道阻滞剂）引起的肺泡液体清除率和肾上腺素清除率的下降幅度大于普萘洛尔（一种非选择性β-肾上腺素能拮抗剂）。虽然普萘洛尔和酚妥拉明（一种α-肾上腺素能拮抗剂）和5-（N-乙基-N-异丙基）阿米洛利（一种Na+/H+反向转运阻滞剂）既不改变肺泡液清除率，也不改变去甲肾上腺素清除率，但阿米洛利和苯扎米尔（一种钠通道阻滞剂）降低了这两种清除率。在大鼠肺中，阿米洛利降低人肺中肺泡液和去甲肾上腺素的清除率。结论：这些结果表明，在大鼠和人的肺中，从肺泡腔中清除儿茶酚胺的速率与肺泡液体清除率相关。
Background: Although aerosolized β-adrenergic agonists have been used as a therapy for the resolution of pulmonary edema, the mechanisms of catecholamine clearance from the alveolar spaces of the lung are not well known. Objective: To determine whether catecholamine clearance from the alveolar spaces is correlated with the fluid transport capacity of the lung. Methods: Albumin solution containing epinephrine (10–7M) or norepinephrine (10–7M) was instilled into the alveolar spaces of isolated rat and human lungs. Alveolar fluid clearance rate was estimated by the progressive increase in the albumin concentration over 1 h. Catecholamine clearance rate was estimated by the changes in catecholamine concentration and alveolar fluid volume over 1 h. Results: The norepinephrine clearance rate was faster than the epinephrine clearance rate in the rat and human lungs. In the rat lungs, amiloride (a sodium channel blocker) caused a greater decrease in alveolar fluid clearance and epinephrine clearance rate than propranolol (a nonselective β-adrenergic antagonist). Although propranolol and phentolamine (an α-adrenergic antagonist), and 5-(N-ethyl-N-isoprophyl)amiloride (a Na+/H+ antiport blocker) changed neither the alveolar fluid clearance nor the norepinephrine clearance rate, amiloride and benzamil (a sodium channel blocker) decreased both clearance rates. As in the rat lungs, amiloride decreased alveolar fluid and norepinephrine clearance rates in the human lungs. Conclusion: These results indicate that the catecholamine clearance rate from the alveolar spaces is correlated with alveolar fluid clearance in rat and human lungs.