Considerations in the analysis of hydrogen exchange mass spectrometry data.
Considerations in the analysis of hydrogen exchange mass spectrometry data.
复制标题
DOI：
10.1007/978-1-62703-392-3_11
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Engen, John R
中科院分区：
文献类型：
--
作者：
Wales, Thomas E;Eggertson, Michael J;Engen, John R
氢交换质谱实验的一个主要组成部分是分析蛋白质和肽质谱，以产生有关氘掺入的信息。对产生的数据的处理包括鉴别每种消化肽，以创建肽序列、保留时间和保留时间范围、质量范围和未氘化质量的主表/阵列。然后必须确定掺入该阵列中的每个肽中的氘的量。已经开发了各种软件平台来执行这种特定类型的数据分析。我们描述的基本参数要考虑在每一步沿着的方式和数据处理，无论是由个人或软件，必须接近分析。
A major component of a hydrogen exchange mass spectrometry experiment is the analysis of protein and peptide mass spectra to yield information about deuterium incorporation. The processing of data that are produced includes the identification of each peptic peptide to create a master table/array of peptide sequence, retention time and retention time range, mass range, and undeuterated mass. The amount of deuterium incorporated into each of the peptides in this array must then be determined. Various software platforms have been developed in order to perform this specific type of data analysis. We describe the fundamental parameters to be considered at each step along the way and how data processing, either by an individual or by software, must approach the analysis.