Relationship between quin2-determined cytosolic [Ca2+] and sweat secretion.
Relationship between quin2-determined cytosolic [Ca2+] and sweat secretion.
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quin2 测定的胞质 [Ca2] 与汗液分泌之间的关系。
DOI：
10.1152/ajpcell.1988.254.2.c310
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Sato,F
中科院分区：
文献类型：
--
作者：
Sato,K;Sato,F
尽管乙酰甲胆碱 (MCh) 和 A23187 诱导的汗液分泌均严格依赖于细胞外 Ca2+，但细胞内钙浓度 ([Ca2+]i) 在小汗腺出汗中的作用尚未阐明。通过连续胶原酶消化和 Percoll 梯度离心，从 400 至 600 个分离的猴汗腺分泌线圈中制备部分纯化的小汗腺分泌细胞。 quin2 方法用于半定量测定 [Ca2+]i，MCh 以剂量依赖性、可逆且药理学特异性的方式增加 [Ca2+]i（从 80-320 nM 的静息 [Ca2+]i），但未能增加不含 Ca2+ 的培养基中的 [Ca2+]i。 A23187 (10(-7) M) 将 [Ca2+]i 增加至约 900 nM。茶碱 (TH)、异丙肾上腺素 (ISO) 和毛喉素 (FK) 对静息 [Ca2+]i 没有影响，但联合使用会减弱随后添加的 MCh 和 A23187 的影响。 10(-7) M 的 A23187 未能在体外分别刺激负载 quin2 的完整分离汗腺和分散的汗液分泌细胞的汗液分泌或 CO2 产生。观察到的 [Ca2+] 增加之间的解离可能表明，除了 [Ca2+] 增加之外，MCh 刺激还会诱导一些未知的兴奋信号，或者在未解离的汗腺中，A23187 诱导的 Ca2+ 流入分泌细胞的量要低得多。
Although both methacholine (MCh)- and A23187-induced sweat secretion are known to be strictly dependent on extracellular Ca2+, the role of intracellular calcium concentration ([Ca2+]i) in eccrine sweating has not been clarified. Partially purified eccrine secretory cells were prepared from 400 to 600 isolated secretory coils of monkey sweat glands by serial collagenase digestion and Percoll gradient centrifugation. The quin2 method was used for semiquantitative determination of [Ca2+]i, MCh increased [Ca2+]i in a dose-dependent, reversible, and pharmacologically specific manner (from the resting [Ca2+]i of 80-320 nM) but failed to increase [Ca2+]i in a Ca2+-free medium. A23187 (10(-7) M) increased [Ca2+]i to approximately 900 nM. Theophylline (TH), isoproterenol (ISO), and forskolin (FK) had no effect on the resting [Ca2+]i but, in combination, attenuated the effect of subsequently added MCh and A23187. A23187 at 10(-7) M failed to stimulate sweat secretion or CO2 production in vitro from the quin2-loaded intact isolated sweat glands and dispersed sweat secretory cells, respectively. The observed dissociation between the increase in [Ca2+] may suggest either that MCh stimulation induces some unknown excitatory signal in addition to a rise in [Ca2+] or that A23187-induced Ca2+ influx into the secretory cells is much lower in undissociated sweat glands.