Cell cycle features of primate embryonic stem cells
Cell cycle features of primate embryonic stem cells
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DOI：
10.1634/stemcells.2005-0194
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发表时间：
2006-03-01
期刊：
影响因子：
5.2
通讯作者：
Dehay, Colette
中科院分区：
文献类型：
--
作者：
Fluckiger, Anne-Catherine;Marcy, Guillaume;Dehay, Colette
利用流式细胞术测量并结合细胞周期动力学的定量分析，我们表明恒河猴胚胎干细胞（ESCs）的特征是极其快速地通过G₁期，G₁期占细胞周期总时长的15%。猴胚胎干细胞呈现细胞周期蛋白E的非阶段性表达，在细胞周期的各个阶段都能检测到，并且在γ射线照射后不会在G₁期生长停滞，这反映出不存在G₁期检查点。血清剥夺或有丝分裂原活化蛋白激酶激酶（MEK）的药理抑制并未导致细胞周期分布发生任何改变，这表明胚胎干细胞的生长不依赖于由Ras/Raf/MEK通路转导的有丝分裂信号。综上所述，这些数据表明恒河猴胚胎干细胞与其小鼠对应物一样，表现出不寻常的细胞周期特征，即G₁期起作用的细胞周期控制机制减弱或缺失。
Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G(1) phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G(1) after gamma-irradiation, reflecting the absence of a G(1) checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G(1) phase are reduced or absent.