Metabolomic Analysis of Serum Glycerophospholipid Levels in Eosinophilic and Neutrophilic Asthma
Metabolomic Analysis of Serum Glycerophospholipid Levels in Eosinophilic and Neutrophilic Asthma
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DOI：
10.3967/bes2019.013
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发表时间：
2019-02-01
影响因子：
3.5
通讯作者：
Zhang Xu
中科院分区：
文献类型：
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作者：
Gai Xiao Yan;Zhang Li Jiao;Zhang Xu
目的通过靶向代谢组学分析比较哮喘炎症亚型的血清甘油磷脂水平。方法收集2015年1月至2015年12月51例哮喘患者的人口学和临床资料，并进行血常规和痰诱导检查。嗜酸性粒细胞性哮喘定义为诱导痰中含有≥3%嗜酸性粒细胞，中性粒细胞性哮喘定义为诱导痰中含有≥71%中性粒细胞。通过液相色谱-质谱法测定血清代谢甘油磷脂谱。采用偏最小二乘判别分析法分析嗜酸性粒细胞与非嗜酸性粒细胞哮喘之间、中性粒细胞与非中性粒细胞哮喘之间甘油磷脂水平的差异。结果痰中嗜酸性粒细胞≥3%组的血清溶血磷脂酰甘油水平显着高于痰中嗜酸性粒细胞<3%组 痰中存在嗜酸性粒细胞。接受者操作特征曲线下面积>= 70%。痰中性粒细胞>=71%组与痰中性粒细胞<71%组之间血清代谢甘油磷脂谱无显着差异。结论 血清中溶血磷脂酰甘油在嗜酸性粒细胞性哮喘中大量产生，可能是嗜酸性粒细胞性哮喘的生物标志物。这些信息有助于识别和定制常见哮喘亚型的治疗方法。
Objective To compare the serum glycerophospholipid levels in the inflammatory subtypes of asthma by using targeted metabolomic analysis.Methods Demographic and clinical data were collected from 51 patients with asthma between January 2015 and December 2015. Routine blood and sputum induction tests were performed. Eosinophilic asthma was defined as induced sputum containing >= 3% eosinophils, and neutrophilic asthma, as induced sputum containing >= 71% neutrophils. Serum metabolic glycerophospholipid profile was determined by liquid chromatography-mass spectrometry. Differences in glycerophospholipid levels between eosinophilic and non-eosinophilic asthma and between neutrophilic and non-neutrophilic asthma were analyzed using partial least squares discriminant analysis.Results The serum lysophosphatidylglycerol level was significantly higher in the group with >= 3% eosinophils in sputum than in the group with < 3% eosinophils in sputum. The area under the receiver-operating characteristic curve was >= 70%. There was no significant difference in the serum metabolic glycerophospholipid profile between the group with sputum neutrophils >= 71% and the group with sputum neutrophils < 71%.Conclusion Serum lysophosphatidylglycerol is produced abundantly in eosinophilic asthma and may be a biomarker of eosinophilic asthma. This information is helpful for identifying and tailoring treatment for the common asthma subtypes.