Viral excretion in cervicovaginal secretions of HIV-1-infected women receiving antiretroviral therapy
Viral excretion in cervicovaginal secretions of HIV-1-infected women receiving antiretroviral therapy
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DOI：
10.1007/s10096-001-8055-2
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发表时间：
2001-02-01
影响因子：
4.5
通讯作者：
Filice, G
中科院分区：
文献类型：
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作者：
Debiaggi, M;Zara, F;Filice, G
进行纵向研究以评估接受抗逆转录病毒治疗的HIV-1血清阳性妇女的宫颈阴道分泌物中存在的病毒脱落。在21个月的中位随访期内，从37名妇女中共获得了128对宫颈阴道和血液样本。一个敏感的，竞争性的，聚合酶链反应和逆转录聚合酶链反应用于同时定量的HIV-1前病毒的DNA和RNA在宫颈阴道细胞和宫颈阴道分泌物中的无细胞RNA，以及HIV-1 RNA在外周血中。随着时间的推移，在生殖器分泌物中检测到前病毒DNA的累积概率在可检测到gE病毒血症的女性中显著高于在血浆中持续检测不到HIV-1 RNA的女性（
A longitudinal study was conducted to evaluate the viral shedding present in cervicovaginal secretions of HIV-l-seropositive women receiving antiretroviral therapy. A total of 128 paired cervicovaginal and blood samples was obtained from 37 women during a median follow-up period of 21 months. A sensitive, competitive, polymerase chain reaction and a reverse transcription polymerase chain reaction were used for the simultaneous quantitation of HIV-1 proviral DNA and RNA in cervicovaginal cells and cell-free RNA in cervicovaginal secretions, as well as HIV-1 RNA in peripheral blood. The cumulative probability of detecting proviral DNA in genital secretions was significantly higher over time in women with detectable ge viremia than in women in whom HIV-1 RNA was persistently undetectable in plasma (