An Optogenetic Method to Modulate Cell Contractility during Tissue Morphogenesis.
An Optogenetic Method to Modulate Cell Contractility during Tissue Morphogenesis.
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DOI：
10.1016/j.devcel.2015.10.020
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发表时间：
2015-12-07
影响因子：
11.8
通讯作者：
De Renzis S
中科院分区：
文献类型：
--
作者：
Guglielmi G;Barry JD;Huber W;De Renzis S
多细胞生物的形态发生是由局部细胞形状变化驱动的。单个细胞或细胞群的行为变化如何以及在多大程度上影响邻近细胞和大规模组织重塑仍然是一个悬而未决的问题。事实上，我们对多细胞动力学的理解是有限的，缺乏方法，允许高时空精度的细胞行为的调制。在这里，我们开发了一种光遗传学方法来实现细胞收缩性的局部调节，并使用它来控制果蝇胚胎发生过程中的形态发生运动。我们发现，局部抑制顶端收缩足以引起全球逮捕中胚层内陷。通过改变内陷过程中抑制的空间模式，我们进一步证明了协调的收缩行为对局部组织几何约束的响应。总之，这些结果显示了这种光遗传学方法在剖析复杂形态发生过程中细胞-细胞相互作用、力传递和组织几何形状之间的相互作用方面的功效。光遗传学提供了一种控制组织形态发生的强有力的方法双光子照明允许光遗传学激活的精确模式细胞收缩性的局部调节揭示了组织内陷的机制组织几何结构限制了细胞收缩行为驱动内陷细胞行为的局部变化如何影响整体组织重塑仍然是一个悬而未决的问题。在这篇参考文献中，Guglielmi et al.提出了一种光遗传学方法，在果蝇胚胎发生过程中局部调节细胞收缩性，并使用它来探测中胚层内陷过程中的细胞-细胞相互作用，力传递和组织几何形状。
Morphogenesis of multicellular organisms is driven by localized cell shape changes. How, and to what extent, changes in behavior in single cells or groups of cells influence neighboring cells and large-scale tissue remodeling remains an open question. Indeed, our understanding of multicellular dynamics is limited by the lack of methods allowing the modulation of cell behavior with high spatiotemporal precision. Here, we developed an optogenetic approach to achieve local modulation of cell contractility and used it to control morphogenetic movements during Drosophila embryogenesis. We show that local inhibition of apical constriction is sufficient to cause a global arrest of mesoderm invagination. By varying the spatial pattern of inhibition during invagination, we further demonstrate that coordinated contractile behavior responds to local tissue geometrical constraints. Together, these results show the efficacy of this optogenetic approach to dissect the interplay between cell-cell interaction, force transmission, and tissue geometry during complex morphogenetic processes. Optogenetics provides a powerful approach to control tissue morphogenesis Two-photon illumination allows precise patterns of optogenetic activation Local modulation of cell contractility reveals mechanisms of tissue invagination Tissue geometry constrains the cell contractile behavior driving invagination How localized changes in cell behavior influence global tissue remodeling remains an open question. In this Resource article, Guglielmi et al. present an optogenetic approach to locally modulate cell contractility during Drosophila embryogenesis and use it to probe cell-cell interaction, force transmission, and tissue geometry during mesoderm invagination.