Design and Synthesis of Gold-Gadolinium-Core-Shell Nanoparticles as Contrast Agent: a Smart Way to Future Nanomaterials for Nanomedicine Applications (Retracted Article)
Design and Synthesis of Gold-Gadolinium-Core-Shell Nanoparticles as Contrast Agent: a Smart Way to Future Nanomaterials for Nanomedicine Applications (Retracted Article)
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DOI：
10.2147/ijn.s224805
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Spadavecchia, Jolanda
中科院分区：
文献类型：
--
作者：
Aouidat, Fatima;Boumati, Sarah;Spadavecchia, Jolanda
简介：用于合成Gd（III）纳米颗粒的生物聚合物的开发，作为治疗剂，可以在纳米医学中发挥关键作用。生物相容性聚合物不仅用于复合单价生物分子，而且还用于实现多价活性靶向材料作为诊断和/或治疗杂化纳米颗粒。本文首次报道了Gd（III）-生物聚合物-Au（III）配合物的合成方法，并将其作为核壳型金纳米粒子（Gd（@AuNPs））的关键成分。材料与方法：采用光谱分析技术（拉曼光谱、紫外-可见光谱和透射电镜）对其进行了理化评价。通过密度泛函理论（DFT）分析的理论表征在特定条件下进行，以研究在第一成核步骤期间Au和Gd前体之间的相互作用。还进行了在7 T下具有弛豫率测量的磁性特征以及用于生物相容性研究的肝细胞系的细胞毒性研究。在小鼠体内详细的动态生物分布研究，以表征生物MRI造影剂的潜在应用，然后achieved.Results：物理化学评价证实了成功的设计和反应假设。使用Alcohol蓝细胞毒性试验，用递增浓度的纳米颗粒评价TIB-75（肝细胞）细胞的活力。然后完成体内生物分布研究，评估纳米颗粒在小鼠体内的动力学行为，并表征其静脉注射后的隐蔽性。结论：我们证明了Gd@AuNPs在肝脏中显示肝细胞方面具有一些优势。特别地，这些纳米缀合物使若干量的Gd@ NP良好地细胞摄取到细胞中，同时保持细胞内的T1对比度，其使用T1加权MR图像提供稳健的体内检测。这些结果将加强钆作为金络合物的作用，以调整Gd（@AuNPs）作为纳米医学领域的创新诊断剂。
Introduction: The development of biopolymers for the synthesis of Gd(III) nanoparticles, as therapeutics, could play a key role in nanomedicine. Biocompatible polymers are not only used for complex monovalent biomolecules, but also for the realization of multivalent active targeting materials as diagnostic and/or therapeutic hybrid nanoparticles. In this article, it was reported for the first time, a novel synthesis of Gd(III)-biopolymer-Au(III) complex, acting as a key ingredient of core-shell gold nanoparticles (Gd(@AuNPs).Material and methods: The physical and chemical evaluation was carried out by spectro-scopic analytical techniques (Raman spectroscopy, UV-visible and TEM). The theoretical characterization by DFT (density functional theory) analysis was carried out under specific conditions to investigate the interaction between the Au and the Gd precursors, during the first nucleation step. Magnetic features with relaxivity measurements at 7T were also performed as well as cytotoxicity studies on hepatocyte cell lines for biocompatibility studies. The in vivo detailed dynamic biodistribution studies in mice to characterize the potential applications for biology as MRI contrast agents were then achieved.Results: Physical-chemical evaluation confirms the successful design and reaction supposed. Viabilities of TIB-75 (hepatocytes) cells were evaluated using Alamar blue cytotoxic tests with increasing concentrations of nanoparticles. In vivo biodistribution studies were then accomplished to assess the kinetic behavior of the nanoparticles in mice and characterize their stealthiness property after intravenous injection.Conclusion: We demonstrated that Gd@AuNPs have some advantages to display hepatocytes in the liver. Particularly, these nanoconjugates give a good cellular uptake of several quantities of Gd@NPs into cells, while preserving a T1 contrast inside cells that provide a robust in vivo detection using T1-weighted MR images. These results will strengthen the role of gadolinium as complex to gold in order to tune Gd(@AuNPs) as an innovative diagnostic agent in the field of nanomedicine.