Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization program
Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization program
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DOI：
10.1016/s0015-0282(97)81915-2
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发表时间：
1997-02-01
影响因子：
6.7
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Nakahara, K;Saito, H;Watanabe, H
目的：探讨壁颗粒中凋亡小体的发生率。设计：非随机、前瞻性研究。单位：日本山形大学医学院妇产科。患者：129名正常排卵的女性接受GnRH类似物（GnRH-a）和促性腺激素的NF-ET诱导排卵。干预措施：患者在给予hCG后进行卵泡抽吸。主要观察指标：荧光显微镜观察恢复颗粒细胞的细胞核，并统计凋亡小体的发生率。结果：129例患者中，壁粒细胞团的凋亡小体发生率明显高于积云细胞团。小于6个卵泡卵母细胞的患者壁粒细胞团和卵丘细胞团的凋亡小体发生率均明显高于大于或等于6个卵母细胞的患者。未妊娠患者壁粒细胞团中凋亡小体的发生率明显高于妊娠患者。结论：壁粒细胞团和积云细胞团在卵泡成熟过程中可能具有不同的功能。壁粒细胞团中凋亡小体的发生率可作为体外受精成功率的指标。
Objective: To investigate the incidence of apoptotio bodies in mural granulosaDesign: Nonrandomized, prospective study.Setting: Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.Patient(s): One hundred twenty-nine normally ovulating women underwent ovulation induction for NF-ET with GnRH analogue (GnRH-a) and gonadotropins.Intervention(s): Patients underwent follicle aspiration after the administration of hCG.Main Outcome Measure(s): The nuclei of recovered granulosa cells were examined by fluorescence microscopy and the incidence of apoptotic bodies was tabulated.Result(s): The incidence of apoptotic bodies was significantly higher in mural granulosa cell masses than in cumulus cell masses in the entire group of 129 patients. Both incidence of apoptotic bodies of mural granulosa cell masses and cumulus cell masses were significantly higher in patients with less than six follicular oocytes compared with patients with six or more oocytes. Nonpregnant patients showed significantly higher incidence of apoptotic bodies in mural granulosa cell masses compared with pregnant patients.Conclusion(s): These results indicate that mural granulosa cell masses and cumulus cell masses may have different functions in follicular maturation. The incidence of apoptotic bodies in mural granulosa cell masses can be used as an indicator of success of IVF.