Bone morphogenetic protein-1: The type I procollagen C-proteinase
Bone morphogenetic protein-1: The type I procollagen C-proteinase
复制标题
DOI：
10.1126/science.271.5247.360
复制
发表时间：
1996-01-19
期刊：
影响因子：
56.9
通讯作者：
Greenspan, DS
中科院分区：
文献类型：
--
作者：
Kessler, E;Takahara, K;Greenspan, DS
骨形态发生蛋白（BMP）是能够诱导异位骨形成的骨源性因子。与其他BMP不同，BMP-1不像转化生长因子-β（TGF-β），但它是一个假定的蛋白酶家族的原型，涉及在不同生物体发育过程中的模式形成。虽然这一组的一些成员，如果蝇tolloid（Drosophila tolloid），被假定为激活TGF-β样蛋白，但实际底物是未知的，前胶原C蛋白酶（PCP）切割前胶原I，II和III的COOH-前肽，产生脊椎动物细胞外基质的主要纤维成分。这里显示BMP-1和PCP是相同的。BMP-1/TLD样蛋白酶活性的证明将参与基质沉积的酶与参与模式形成的基因联系起来。
Bone morphogenetic proteins (BMPs) are bone-derived factors capable of inducing ectopic bone formation. Unlike other BMPs, BMP-1 is not like transforming growth factor-beta (TGF-beta), but it is the prototype of a family of putative proteases implicated in pattern formation during development in diverse organisms. Although some members of this group, such as Drosophila tolloid (TLD), are postulated to activate TGF-beta-like proteins, actual substrates are unknown, Procollagen C-proteinase (PCP) cleaves the COOH-propeptides of procollagens I, II, and III to yield the major fibrous components of vertebrate extracellular matrix. Here it is shown that BMP-1 and PCP are identical. This demonstration of enzymatic activity for a BMP-1/TLD-like protein links an enzyme involved in matrix deposition to genes involved in pattern formation.