Exosomal natural antisense transcripts in culture supernatants released from colorectal cancer SW480 cells
Exosomal natural antisense transcripts in culture supernatants released from colorectal cancer SW480 cells
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DOI：
10.4066/biomedicalresearch.29-18-272
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发表时间：
2018
影响因子：
1.2
通讯作者：
M. Chiba
中科院分区：
文献类型：
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作者：
M. Chiba
简介：外泌体是细胞外囊泡，由大多数细胞释放，参与细胞间通讯。外泌体含有膜和胞质成分，例如蛋白质、脂质、信使核糖核酸 (mRNA) 和 microRNA。然而，这些外泌体中是否存在其他类型的RNA尚不清楚。方法：在本研究中，使用有义/反义定制微阵列研究了结直肠癌 SW480 细胞释放的外泌体中基因的反向链脱氧核酸转录的自然反义转录本 (NAT) 的存在。结果与讨论：当选择大于100的信号强度时，在SW480细胞释放的外泌体中检测到6,218个NAT。其中，使用链特异性逆转录聚合酶链式反应检测从转录因子7-like 1基因转录的NAT，其信号强度最低。因此，大量的 NAT 以及 mRNA 和 microRNA 存在于外泌体中，并且可能被递送到其他细胞。
Introduction: Exosomes are extracellular vesicles, which are released from most cells, involved in cell-tocell communication. Exosomes contain membrane and cytosolic components such as proteins, lipids, messenger Ribonucleic Acids (mRNAs), and microRNAs. However, the existence of other types of RNAs in these exosomes is unknown. Methods: In the present study, the presence of Natural Antisense Transcripts (NATs) transcribed from reverse strand deoxynucleic acids of genes in exosomes released from colorectal cancer SW480 cells were investigated using a sense/antisense custom microarray. Results and Discussion: When signal intensities with values of more than 100 were selected, 6,218 NATs were detected in exosomes released from SW480 cells. Among them, NATs transcribed from transcription factor 7-like 1 genes, which had the lowest signal intensities, were detected using strandspecific reverse transcription-polymerase chain reaction. Thus, a large number of NATs as well as mRNAs and microRNAs are present in exosomes and are likely to be delivered to other cells.