Fine mapping and candidate gene prediction of a pleiotropic quantitative trait locus for yield-related trait in Zea mays.
Fine mapping and candidate gene prediction of a pleiotropic quantitative trait locus for yield-related trait in Zea mays.
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DOI：
10.1371/journal.pone.0049836
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Liu R;Jia H;Cao X;Huang J;Li F;Tao Y;Qiu F;Zheng Y;Zhang Z
玉米籽粒产量是一个高度复杂的数量性状，受多个效应较小的多个数量性状基因座控制，常受多种遗传和环境因素的影响。因此，在玉米基因组中克隆一个影响籽粒产量的QTL是具有挑战性的。以前，我们在多个环境中定位了一个控制每行粒数(KNPR)的主效QTL qKNPR6，并育成了两个近等基因系SL57-6和Ye478，它们只是在含有该QTL的短片段上的等位基因构成上存在差异。最近，在SL57-6×野478分离群体中对qKNPR6进行了重新评估，并缩小到2.8 cM的间隔，解释了201个F2：3家系KNPR表型变异的56.3%。该QTL同时影响穗长、粒重和产量。此外，一个拥有超过12,800株植物、191条重组染色体和10个重叠重组系的F2群体将qKNPR6放入一个0.91 cM的区间，相当于B73参考基因组的198kb。在该区域，6个带有表达序列标签(EST)证据的基因被注释。对这6个基因在野菜478和SL57-6中的表达模式和DNA多样性进行了分析。讨论了可能的候选基因和参与花序发育的途径。
The yield of maize grain is a highly complex quantitative trait that is controlled by multiple quantitative trait loci (QTLs) with small effects, and is frequently influenced by multiple genetic and environmental factors. Thus, it is challenging to clone a QTL for grain yield in the maize genome. Previously, we identified a major QTL, qKNPR6, for kernel number per row (KNPR) across multiple environments, and developed two nearly isogenic lines, SL57-6 and Ye478, which differ only in the allelic constitution at the short segment harboring the QTL. Recently, qKNPR6 was re-evaluated in segregating populations derived from SL57-6×Ye478, and was narrowed down to a 2.8 cM interval, which explained 56.3% of the phenotypic variance of KNPR in 201 F2∶3 families. The QTL simultaneously affected ear length, kernel weight and grain yield. Furthermore, a large F2 population with more than 12,800 plants, 191 recombinant chromosomes and 10 overlapping recombinant lines placed qKNPR6 into a 0.91 cM interval corresponding to 198Kb of the B73 reference genome. In this region, six genes with expressed sequence tag (EST) evidence were annotated. The expression pattern and DNA diversity of the six genes were assayed in Ye478 and SL57-6. The possible candidate gene and the pathway involved in inflorescence development were discussed.
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影响因子：
5.4
作者：
Messmer, Rainer;Fracheboud, Yvan;Ribaut, Jean-Marcel
通讯作者：
Ribaut, Jean-Marcel
DOI：
10.1073/pnas.172520999
发表时间：
2002-10-15
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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