Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
复制标题
DOI：
10.1083/jcb.108.3.885
复制
发表时间：
1989-03
影响因子：
7.8
通讯作者：
Capco, D G
中科院分区：
文献类型：
--
作者：
Bement, W M;Capco, D G
使用蛋白激酶 C 激活剂、佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA)、佛波醇 12,13-二癸酸酯和 1-油酰基-2-乙酰基-sn-甘油对不含卵泡细胞的前期 I 卵母细胞和中期 II 卵进行短暂处理。在卵母细胞和卵中，这些处理触发了两栖动物发育的早期事件：皮质颗粒胞吐作用、皮质收缩和卵裂沟形成。令人惊讶的是，卵母细胞的激活发生在没有减数分裂恢复的情况下，导致细胞具有卵母细胞样核和内部细胞质，但具有合子样皮质。 PMA诱导的卵母细胞和卵子的激活不需要外部钙，这是卵子正常激活的先决条件。 PMA 诱导的卵子激活被视黄酸（一种已知的蛋白激酶 C 抑制剂）抑制。此外，用视黄酸预处理卵子可防止机械刺激引起的激活并抑制钙离子载体 A23187 的激活。结果表明蛋白激酶 C 激活是非洲爪蟾受精途径的一个组成部分。
Prophase I oocytes, free of follicle cells, and metaphase II eggs of the amphibian Xenopus laevis were subjected to transient treatments with the protein kinase C activators, phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-didecanoate, and 1-olyeoyl-2-acetyl-sn-glycerol. In both oocytes and eggs, these treatments triggered early events of amphibian development: cortical granule exocytosis, cortical contraction, and cleavage furrow formation. Surprisingly, activation of oocytes occurred in the absence of meiotic resumption, resulting in cells with an oocytelike nucleus and interior cytoplasm, but with a zygotelike cortex. PMA-induced activation of oocytes and eggs did not require external calcium, a prerequisite for normal activation of eggs. PMA-induced activation of eggs was inhibited by retinoic acid, a known inhibitor of protein kinase C. In addition, pretreatment of eggs with retinoic acid prevented activation by mechanical stimulation and inhibited activation by calcium ionophore A23187. The results suggest that protein kinase C activation is an integral component of the Xenopus fertilization pathway.