Mutual anti-oxidative effect of gossypol acetic acid and gossypol-iron complex on hepatic lipid peroxidation in male rats
Mutual anti-oxidative effect of gossypol acetic acid and gossypol-iron complex on hepatic lipid peroxidation in male rats
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DOI：
10.1016/j.fct.2009.08.001
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发表时间：
2009-11-01
影响因子：
4.3
通讯作者：
El-Shahawy, I. N.
中科院分区：
文献类型：
--
作者：
El-Sharaky, A. S.;Wahby, M. M.;El-Shahawy, I. N.
棉酚在男性中具有抗癌和抗生育作用。雄性大鼠分别用棉酚乙酸（GAA）或棉酚铁复合物（GIC）治疗。血清谷丙转氨酶（ALT）活性升高。然而，gic处理的动物肝脏谷胱甘肽（GSH）含量下降，丙二醛（MDA）含量增加。而GAA组GSH-Px特异性活性升高。GAA和GIC诱导肝脏NEFA显著升高，总饱和脂肪酸显著降低，PUFA显著升高。棉酚可以抑制脂质过氧化，从而保护脂质免受氧化损伤。酚类被氧化为苯氧自由基，由于共振稳定而不允许抗氧化。GAA刺激羟基自由基（(OH)- o -中心点）的产生和DNA损伤。硫代巴比妥酸活性物质（TBARS）的急剧增加证明了GAA和GIC会增加脂质过氧化。据报道，TBARS对MDA以外的化合物的特异性存在争议。如果TBARS增加，则需要采用更具体的检测方法。脂质分类和脂肪酸模式的测定，揭示了该技术在评估组织脂质过氧化的意义。GAA和GIC是有效的脂质过氧化抑制剂，具有促氧化和抗氧化作用，而GIC的毒性较小。2009爱思唯尔有限公司版权所有。
Gossypol displays anticancer behavior and anti-fertility in males. Male rats were treated with either gossypol acetic acid (GAA) or gossypol-iron complex (GIC). Serum alanine transaminase (ALT) activity elevated of GAA. However, GIC-treated animals showed a decrease in hepatic glutathione (GSH) content with increased malondialdehyde (MDA) content. Whereas, GSH-Px specific activity increased in GAA group. GAA and GIC induce significant increases in the hepatic NEFA with remarkable decrease in the total saturated fatty acids with a significant increase of PUFA.Lipid peroxidation is inhibited by gossypol, which shield lipids against oxidative damage. Phenols are oxidized to phenoxy radicals, which do not permit anti-oxidation due to resonance stabilization. GAA stimulate hydroxyl radicals ((OH)-O-center dot) generation and DNA damage. GAA and GIC produce increase in lipid peroxidation as proved by a steep rise in thiobarbituric acid reactive species (TBARS). Controversy of specificity of TBARS towards compounds other than MDA was reported. If TBARS increased, more specific assay to be employed. Assay of lipid classes and fatty acids pattern, reveled the significance of the technique in assessment of lipid peroxidation in tissues. GAA and GIC were powerful inhibitors of lipid peroxidation and exhibit pro- and antioxidant behavior, with less toxicity of GIC. (C) 2009 Elsevier Ltd. All rights reserved.