First evidence of the possible implication of the 11-deoxycorticosterone (DOC) in immune activity of Eurasian perch (Perca fluviatilis, L.): Comparison with cortisol
First evidence of the possible implication of the 11-deoxycorticosterone (DOC) in immune activity of Eurasian perch (Perca fluviatilis, L.): Comparison with cortisol
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DOI：
10.1016/j.cbpa.2013.02.025
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发表时间：
2013-06-01
影响因子：
2.3
通讯作者：
Kestemont, Patrick
中科院分区：
文献类型：
--
作者：
Mathieu, Cedric;Milla, Sylvain;Kestemont, Patrick
皮质醇是鱼类体内主要的皮质类固醇激素，常被描述为鱼类免疫系统的调节剂。此外，11-脱氧皮质酮（DOC）被证明可以结合和转录激活盐皮质激素受体，并可能作为一种盐皮质激素在鱼。这两种皮质类固醇的腹腔注射诱导的免疫调节在欧亚鲈鱼少年进行了评估。皮质醇和DOC分别以0.8mg kg（-1）和0.08mg kg（-1）体重注射。皮质醇增加血浆溶菌酶活性注射后72小时，C型溶菌酶在脾脏中的表达从注射后1至72小时，并有利于血液中的中性粒细胞的淋巴细胞和血小板的混合物的费用。此外，注射后6小时，皮质醇降低脾脏中促炎细胞因子TNF-α的表达水平。DOC对血浆中的免疫指标没有影响，但增加了鳃和脾中C型溶菌酶和载脂蛋白A1 mRNA的表达水平。同时，DOC通过增加脾中盐皮质激素受体和11 β-羟基类固醇脱氢酶-2的表达来刺激其假定的信号通路。这些结果证实了皮质醇作为一种先天的短期免疫刺激剂的作用。DOC首次被描述为鱼类可能的免疫刺激剂。(C)2013 Elsevier Inc. All rights reserved.
Cortisol, the main corticosteroid in fish, is frequently described as a modulator of fish immune system. Moreover, 11-deoxycorticosterone (DOC) was shown to bind and transcriptionally activate the mineralocorticoid receptor and may act as a mineralocorticoid in fish. Immune modulations induced by intraperitoneal injections of these two corticosteroids were assessed in Eurasian perch juveniles. Cortisol and DOC were injected at 0.8 mg kg(-1) and 0.08 mg kg(-1) body weight respectively. Cortisol increased plasma lysozyme activity 72 h post-injection, C-type lysozyme expression in spleen from 1 to 72 h post-injection, and favoured blood neutrophils at the expense of a mixture of lymphocytes and thrombocytes. Moreover, 6 h after injection, cortisol reduced expression levels of the pro-inflammatory cytokine TNF-alpha in spleen. DOC had no effects on the immune variables measured in plasma, but increased expression levels of C-type lysozyme and apolipoprotein A1 mRNA in both gills and spleen. Meanwhile, DOC stimulated its putative signalling pathway by increasing expression of mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase-2 in spleen. These results confirmed the role of cortisol as an innate, short term immune stimulator. For the first time, DOC is described as a possible immune stimulator in fish. (C) 2013 Elsevier Inc. All rights reserved.