FELINES: a utility for extracting and examining EST-defined introns and exons
FELINES: a utility for extracting and examining EST-defined introns and exons
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DOI：
10.1093/nar/gng141
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发表时间：
2003-11-15
影响因子：
14.9
通讯作者：
Murphy, JW
中科院分区：
文献类型：
--
作者：
Drabenstot, SD;Kupfer, DM;Murphy, JW
FELINES（查找和检查大量内含子“N”外显子序列）是一个实用程序，用于自动构建和分析从 EST（表达序列标签）到基因组序列比对生成的高质量内含子和外显子序列数据库。我们通过 EST 与基因组序列的比对，为真菌裂殖酵母 (Schizosaccharomyces pombe) 创建内含子和外显子序列数据库，展示了 FELINES 实用程序的各种程序。此外，我们还分析了我们构建的粟酒裂殖酵母序列数据库和 Manuel Ares 实验室完善的酿酒酵母内含子数据库的保守序列基序。 FELINES 被证明可用于表征内含子数据库中的分支位点、多嘧啶束以及 5' 和 3' 剪接位点以及裂殖酵母外显子中的外显子剪接增强子 (ESE)。 FELINES 可在 http://www.genome.ou.edu/informatics.html 上找到。
FELINES (Finding and Examining Lots of Intron 'N' Exon Sequences) is a utility written to automate construction and analysis of high quality intron and exon sequence databases produced from EST (expressed sequence tag) to genomic sequence alignments. We demonstrated the various programs of the FELINES utility by creating intron and exon sequence databases for the fungal organism Schizosaccharomyces pombe from alignments of EST to genomic sequences. In addition, we analyzed our constructed S.pombe sequence databases and the well-established Saccharomyces cerevisiae intron database from Manuel Ares' Laboratory for conserved sequence motifs. FELINES was shown to be useful for characterizing branchsites, polypyrimidine tracts and 5' and 3' splice sites in the intron databases and exonic splicing enhancers (ESEs) in S.pombe exons. FELINES is available at http://www.genome.ou.edu/informatics.html.