Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.
Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.
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使用十八硅基二氧化硅提取和纯化生物样品中的肽。
作者：
H. Bennett;A. Hudson;C. McMartin;G. Purdon
多肽可以从大量的水溶液中吸附在十八烷基硅基硅胶上，并用含有甲醇或乙腈的混合水溶剂洗脱。这些性质可用于从大鼠血浆样品中提取和纯化多肽片段。静脉注射Synacten[促肾上腺皮质激素-(1-24)-四肽]后，在2分钟内循环的主要产物为完整的多肽及其亚硫氧化物。此外，还存在一些表明N-末端和C-末端发生切割的片段。合成产物中的大部分生物活性较低。生长抑素在体内和体外被迅速裂解成单一产物，很可能保留了生物活性。没有其他循环产物表明，生长抑素只有在离开循环后才会失活。
Peptides can be adsorbed on octadecasilyl-silica from large volumes of aqueous solution and eluted with aqueous solvent mixtures containing methanol or acetonitrile. These properties may be used for the extraction and purification of peptide fragments in plasma samples collected from rats. After intravenous injection of Synacthen [corticotropin-(1-24)-tetracosapeptide], it was shown that within 2 min the main circulating products were intact peptide and its sulphoxide. In addition, a number of fragments indicative of cleavage at the N- and C-termini were present. Most of the products formed from Synacthen have low biological activity. Somatostatin was rapidly cleaved in vivo and in vitro to a single product, which probably retains biological activity. The absence of other circulating products suggests that somatostatin is only inactivated once it leaves the circulation.