Expression of CD64 on polymorphonuclear neutrophils in patients with familial Mediterranean fever
Expression of CD64 on polymorphonuclear neutrophils in patients with familial Mediterranean fever
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DOI：
10.1111/j.1365-2249.2011.04380.x
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发表时间：
2011-06-01
影响因子：
4.6
通讯作者：
Eguchi, K.
中科院分区：
文献类型：
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作者：
Migita, K.;Agematsu, K.;Eguchi, K.
家族性地中海热（FMF）是一种自身炎症性疾病，其特征是反复发作的发热和血清或滑膜炎症。我们研究了多形核中性粒细胞（PMN）中CD 64（Fc γ RI）表达作为FMF患者临床和生物学参数的实用性。我们研究了12名日本FMF患者（平均年龄; 22个中心点8 +/- 15个中心点5岁，男性/女性：2/10），沿着类风湿关节炎患者（RA，n = 38例男性/女性：6/32例，平均年龄; 52个中心点2 +/- 15个中心点3岁），系统性红斑狼疮（SLE，n = 15名男性/女性：0/15，平均年龄; 38个中心点5 +/-15个中心点9岁）和12名健康受试者（男性/女性：3/9，平均年龄; 37个中心点9 +/-17个中心点2岁）。使用流式细胞术测定PMN上的CD 64表达。FMF患者CD 64的定量表达（2439中心点6 +/-2215中心点8个分子/PMN）显著高于健康受试者（547中心点8 +/- 229中心点5，P = 0中心点003）或RA患者（606中心点5 +/-228中心点2，P < 0中心点0001）和SLE（681中心点3 +/-281中心点1，P = 0中心点004）。未经治疗的FMF患者分离的PMN上增加的CD 64表达被秋水仙素治疗下调。使用MurNAc-（L）-Ala-（D）-isoGln（MDP）活化含NACHT-LRR-PYD的蛋白3（NLRP 3）导致来自健康受试者的PMN上的CD 64表达增加。我们的研究结果表明，CD 64在中性粒细胞表达的定量测量可以是一个有价值的工具，以区分FMF和自身免疫性疾病。
P>Familial Mediterranean fever (FMF) is an autoinflammatory disease characterized by recurrent episodes of fever and serosal or synovial inflammation. We examined the utility of CD64 (Fc gamma RI) expression in polymorphonuclear neutrophils (PMNs) as clinical and biological parameters in patients with FMF. We studied 12 Japanese FMF patients (mean age; 22 center dot 8 +/- 15 center dot 5 years, male/female: 2/10), along with rheumatoid arthritis patients (RA, n = 38 male/female: 6/32, mean age; 52 center dot 2 +/- 15 center dot 3 years), systemic lupus erythematosus (SLE, n = 15 male/female: 0/15, mean age; 38 center dot 5 +/- 15 center dot 9 years) and 12 healthy subjects (male/female: 3/9, mean age; 37 center dot 9 +/- 17 center dot 2 years). CD64 expression on PMNs was determined using flow cytometry. The quantitative expression of CD64 in patients with FMF (2439 center dot 6 +/- 2215 center dot 8 molecules per PMN) was significantly higher than in healthy subjects (547 center dot 8 +/- 229 center dot 5, P = 0 center dot 003) or in patients with RA (606 center dot 5 +/- 228 center dot 2, P < 0 center dot 0001) and SLE (681 center dot 3 +/- 281 center dot 1, P = 0 center dot 004). The increased CD64 expression on PMNs isolated from untreated FMF patients was down-regulated by colchicine treatment. NACHT-LRR-PYD-containing protein 3 (NLRP3) activation using MurNAc-(L)-Ala-(D)-isoGln (MDP) resulted in increased CD64 expression on PMNs from healthy subjects. Our results suggest that quantitative measurement of CD64 expression on PMNs can be a valuable tool to discriminate between FMF and autoimmune diseases.