Effect of poly(2-methoxyethyl acrylate)-coated oxygenators on haemolysis
Effect of poly(2-methoxyethyl acrylate)-coated oxygenators on haemolysis
复制标题
聚（丙烯酸 2-甲氧基乙酯）涂层充氧器对溶血的影响
DOI：
10.1163/156856206776374098
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
H. Ayhan
中科院分区：
文献类型：
--
作者：
M. Kocakulak;T. Özgürtaş;H. Ayhan
血液与人工器械表面的接触和机械创伤是溶血的两个主要因素。聚（丙烯酸2-甲氧基乙酯）（PMEA）是一种两亲性聚合物，其聚乙烯链是疏水性的，并且具有温和的亲水性尾部。在先前的研究中，PMEA涂层显示出对蛋白质吸附、血小板损失、血小板聚集和术后出血的积极作用。在这项研究中，聚（2-甲基丙烯酸乙酯）（PMEA）涂层氧合器对溶血的影响进行了研究。使用PMEA涂层（SX 18-Capiox）氧合器。研究了氧合器纤维样品中脱附红细胞、游离血红蛋白、间接胆红素和总胆红素的含量。在心肺旁路（CPB）期间的5个不同时间采集血液等分试样，测量红细胞、总胆红素和直接胆红素值;基线（T1）、CPB期间（T2）、CPB结束时（T3）、鱼精蛋白注射后（T4）和重症监护（T5）。在涂层和非涂层氧合器中，溶血率均在临床可接受的安全范围内。平均解吸游离血红蛋白为6663毫克/分升涂层和29.405毫克/分升非涂层纤维。平均解吸总胆红素为0.0068毫克/分升从涂层和0.023毫克/分升从非涂层纤维。我们观察到较少的溶血，这反映在涂层氧合器的脱附游离血红蛋白和间接胆红素较低以及血液红细胞数量减少较少。与对照组相比，涂层组的血液胆红素浓度较低。本研究描述了PMEA涂层与血液接触表面溶血之间的关系。PMEA涂层减少了体外循环过程中红细胞的损伤。
Blood contact with artificial device surfaces and mechanical trauma are two major factors for haemolysis. Poly(2-methoxyethyl acrylate) (PMEA) is an amphiphilic polymer with a polyethylene chain that is hydrophobic and a mildly hydrophilic tail. PMEA coating has showed positive effects on protein adsorption, platelet loss, platelet aggregation and post-operative bleeding in previous studies. In this study, effects of poly(2-metoxyethyl acrylate) (PMEA)-coated oxygenators on haemolysis was investigated. PMEA-coated (SX18-Capiox) oxygenators were used. Desorbed erythrocyte, free haemoglobin indirect bilirubin and total bilirubin quantities from fibre samples of oxygenators were studied. Erythrocyte, total bilirubin and direct bilirubin values were measured from blood aliquots taken in five different times during cardiopulmonary by-pass (CPB); baseline (T1), during CPB (T2), at the end of CPB (T3), after protamine injection (T4) and in intensive care (T5). In both coated and non-coated oxygenators haemolysis rate was in clinically acceptable safety range. Average desorbed free haemoglobin was 6663 mg/dl from coated and 29.405 mg/dl from non-coated fibres. Average desorbed total bilirubin was 0.0068 mg/dl from coated and 0.023 mg/dl from non-coated fibres. We observed less haemolysis, as reflected by lower desorbed free haemoglobin and indirect bilirubin from coated oxygenators and less decrease in blood erythrocyte number. Blood bilirubin concentration was low in the coated group when compared to the control group. This study describes the relationship between PMEA coating and haemolysis at the blood contacting surface. PMEA coating reduces red blood cell damage during extracorporeal circulation.