Occludin is concentrated at tight junctions of mouse/rat but not human guinea pig Sertoli cells in testes
Occludin is concentrated at tight junctions of mouse/rat but not human guinea pig Sertoli cells in testes
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DOI：
10.1152/ajpcell.1998.274.6.c1708
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发表时间：
1998-06-01
影响因子：
5.5
通讯作者：
Tsukita, S
中科院分区：
文献类型：
--
作者：
Moroi, S;Saitou, M;Tsukita, S
Occludin 是迄今为止唯一被确定为紧密连接 (TJ) 组成部分的完整膜蛋白。在这里，我们检查了 Occludin 在具有发育良好的 TJ 的小鼠睾丸中的分布和表达。在成年小鼠睾丸中，occludin 集中在 TJ 链上，该链位于支持细胞侧膜的最基底区域。在免疫印迹中，occludin 显示出特征性的多重条带模式，表明 occludin 在睾丸中高度磷酸化。在1周龄小鼠睾丸中，occludin广泛分布在支持细胞的侧膜上，即使在这个阶段，也检测到高度磷酸化的occludin。随着发育，occludin逐渐集中在支持细胞的最基底区域。在大鼠中获得了相同的结果，但出乎意料的是，通过免疫荧光显微镜以及免疫印迹法，在人或豚鼠支持细胞中没有检测到 occludin。由于 TJ 在这些物种的支持细胞中也发育良好，我得出的结论是，至少在这些物种的睾丸中，存在一些支持细胞特异性的 occludin 亚型或其他与 occludin 不同的 TJ 相关整合膜蛋白。
Occludin is the only integral membrane protein identified to date as a component of tight junctions (TJs). Here, ave examined the distribution and expression of occludin in murine testis bearing well-developed TJ. In the adult mouse testis, occludin was concentrated at TJ strands, which are located at the most basal regions of lateral membranes of Sertoli cells. In immunoblotting, occludin showed a characteristic multiple banding pattern, suggesting that occludin is highly phosphorylated in the testis. In 1-wk-old mouse testis, occludin was distributed diffusely at the lateral membranes of Sertoli cells, and even at this stage, highly phosphorylated occludin was detected. With development, occludin gradually became concentrated at the most basal regions of Sertoli cells. The same results were obtained in rat, but unexpectedly occludin was not detected in human or guinea pig Sertoli cells by immunofluorescence microscopy as well as by immunoblotting. Inasmuch as TJs are also well developed in Sertoli cells of these species, me concluded that, at least in the testes of these species, there are some Sertoli cell-specific isoforms of occludin or other TJ-associated integral membrane proteins that differ from occludin.