Release of nitric oxide from a spin trap, N-tert-butyl-alpha-phenylnitrone, under various oxidative conditions.
Release of nitric oxide from a spin trap, N-tert-butyl-alpha-phenylnitrone, under various oxidative conditions.
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在各种氧化条件下，自旋陷阱 N-叔丁基-α-苯基硝酮释放一氧化氮。
DOI：
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发表时间：
1998
影响因子：
2
通讯作者：
R. Cutler
中科院分区：
文献类型：
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作者：
K. Saito;H. Yoshioka;S. Kazama;R. Cutler
研究了自旋捕获剂N-叔丁基-α-苯基硝酮（PBN）在不同氧化条件下产生一氧化氮（NO）的情况。PBN在295 nm处的吸光度随紫外光照射时间的延长而降低，表明PBN被紫外光照射分解。由芬顿试剂形成的羟基自由基也分解PBN，但由过氧化氢自由基和超氧化物的影响很小。利用Griess反应原理，采用NOx分析仪测定PBN溶液中NO的氧化产物亚硝酸盐。紫外线照射和羟基自由基也形成亚硝酸盐。使用GC/MS/SIM对UV照射的样品进行直接检测，成功地从与羟基自由基反应的样品中检测出NO。PBN溶液中产生的NO激活鸟苷酸环化酶。因此，PBN有望成为一种新型的抗氧化剂和NO供体。
Nitric oxide (NO) generation from a spin trap, N-tert-butyl-alpha-phenylnitrone (PBN) under various oxidative conditions was examined. The absorbance of PBN at 295 nm decreased with time of UV-irradiation, showing that PBN was decomposed by UV irradiation. The hydroxyl radical formed from a Fenton reagent also decomposed PBN, but there was little effect by a peroxyl radical and a superoxide. Nitrite, an oxidative product of NO, in PBN solution was determined using a NOx analyzer based on Griess reaction. UV-irradiation and the hydroxyl radical also formed nitrite. Direct detection of NO from the sample on reaction with hydroxyl radical was successful using a GC/MS/SIM on the UV-irradiated sample. NO generated in PBN solutions activated guanylate cyclase. From these results, PBN is viewed as a new kind of medicine which acts as an antioxidant and as an NO donor in vivo.