Miltefosine inhibits Candida albicans and non-albicans Candida spp. biofilms and impairs the dispersion of infectious cells.
Miltefosine inhibits Candida albicans and non-albicans Candida spp. biofilms and impairs the dispersion of infectious cells.
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DOI：
10.1016/j.ijantimicag.2016.07.022
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发表时间：
2016-11
影响因子：
10.8
通讯作者：
T. Vila;K. Ishida;S. Seabra;S. Rozental
中科院分区：
文献类型：
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作者：
T. Vila;K. Ishida;S. Seabra;S. Rozental
你好可以粘附并在不同表面上形成生物膜，变得对抗真菌治疗不敏感。抗真菌剂的生物膜的耐药性是多因素的，细胞外基质（ECM）似乎发挥了重要作用。在用于治疗念珠菌血症的几种可用的抗真菌剂中，只有阿替霉素B（AmB）和棘白菌素的脂质制剂对生物膜有效。我们的小组先前已经证明米替福新对白色念珠菌生物膜有重要作用。因此，这项工作的目的是扩大米替福新的体外抗菌膜活性的分析，以非白色念珠菌的Escherichia daspp。米替福新对嗜酸性细胞和念珠菌生物被膜的形成有明显的抑制作用。白色念珠菌、近平滑念珠菌、热带念珠菌和光滑念珠菌。在生物膜中的活性特征上级于氟康唑，与AmB和卡泊芬净相似。具有提取的ECM的生物膜衍生的细胞变得与抗肿瘤细胞一样对米替福新敏感，证实了ECM在生物膜抗性行为中的重要性。米替福新还在抑制细胞增殖所需的相同浓度下抑制细胞的生物膜分散。在这项工作中获得的数据加强了米替福新对四种最致病的daspp生物膜的有效抑制活性。并鼓励进一步研究这种药物和/或结构类似物在生物膜相关感染中的应用。
Candidaspp. can adhere to and form biofilms over different surfaces, becoming less susceptible to antifungal treatment. Resistance of biofilms to antifungal agents is multifactorial and the extracellular matrix (ECM) appears to play an important role. Among the few available antifungals for treatment of candidaemia, only the lipid formulations of amphotericin B (AmB) and the echinocandins are effective against biofilms. Our group has previously demonstrated that miltefosine has an important effect againstCandida albicansbiofilms. Thus, the aim of this work was to expand the analyses of the in vitro antibiofilm activity of miltefosine to non-albicans Candidaspp. Miltefosine had significant antifungal activity against planktonic cells and the development of biofilms ofC. albicans,Candida parapsilosis,Candida tropicalisandCandida glabrata. The activity profile in biofilms was superior to fluconazole and was similar to that of AmB and caspofungin. Biofilm-derived cells with their ECM extracted became as susceptible to miltefosine as planktonic cells, confirming the importance of the ECM in the biofilm resistant behaviour. Miltefosine also inhibited biofilm dispersion of cells at the same concentration needed to inhibit planktonic cell growth. The data obtained in this work reinforce the potent inhibitory activity of miltefosine on biofilms of the four most pathogenicCandidaspp. and encourage further studies for the utilisation of this drug and/or structural analogues on biofilm-related infections.