Relationship between hydrocarbons and molecular phylogeny of Botryococcus braunii
Relationship between hydrocarbons and molecular phylogeny of Botryococcus braunii
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DOI：
10.1016/j.algal.2012.05.003
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发表时间：
2012-10-01
影响因子：
5.1
通讯作者：
Watanabe, Makoto M.
中科院分区：
文献类型：
--
作者：
Kawachi, Masanobu;Tanoi, Takako;Watanabe, Makoto M.
利用日本分离的31株无菌菌株，研究了烃类与布朗葡萄球菌分子生物学的关系。由此产生的18 S rRNA树，其中包括来自基因库的额外序列，揭示了三个主要的系统发育分支（I，II和III）和四个亚分支（II 1，II 2，III 1和III 2）的存在，具有较高的自举值。通过气相色谱/质谱联用分析，共检测到9种烃类物质，并将其分为4类：A、B和L 3个已知小种以及暂命名为S的小种，它们分别由碳数为18和20的环氧正构烷烃和饱和正构烷烃组成。进化枝I的菌株与A小种一致。进化枝II含有小种B，进化枝III含有小种L及其衍生物和小种S。进化枝III进一步分为含有典型小种L菌株的亚进化枝III 1和含有小种S的亚进化枝III 2，具有高的自举值。葡萄球菌属菌株的系统发育关系似乎与独特的烃合成途径相当一致。18 S rRNA的最大分歧值表明，在几乎物种水平上，进化枝之间存在大的物种形成，这表明生物化学性质的实用性，例如，烃类类型，在确定葡萄藻属的分类。(c)2012 Elsevier B. V.保留所有权利。
The relationship between hydrocarbons and the molecular phylogeny of Botryococcus braunii was investigated using 31 axenic strains isolated in Japan. The resulting 18S rRNA tree, which includes additional sequences from the gene bank, revealed the existence of three major pylogenetic clades (I, II, and III) and four subclades (II1, II2, III1, and III2) with high bootstrap values. By gas chromatography/mass spectrometry analysis, nine hydrocarbon species were detected and categorized as 4 types: the three known races A, B, and L and the tentatively named race S comprising epoxy-n-alkane and saturated n-alkane chains with carbon numbers 18 and 20, respectively. Strains of clade I were coincident with race A. Clade II contained race B, and clade III contained race L and its derivatives, and race S. Clade III was further categorized into subclade III1 containing the typical race L strains and subclade III2 containing race S with high bootstrap values. The phylogenetic relationship of Botryococcus strains appeared to be in considerable agreement with unique hydrocarbon synthesis pathways. Maximum divergence values of 18S rRNA indicated large speciation among the clades at almost the species level, suggesting the utility of biochemical properties, e.g., hydrocarbon types, in determining the taxonomy of Botryococcus. (c) 2012 Elsevier B.V. All rights reserved.