Successive passaging of the scrapie strains, ME7-ha and 139A-ha, generated by the interspecies transmission of mouse-adapted strains into hamsters markedly shortens the incubation times, but maintains their molecular and pathological properties
Successive passaging of the scrapie strains, ME7-ha and 139A-ha, generated by the interspecies transmission of mouse-adapted strains into hamsters markedly shortens the incubation times, but maintains their molecular and pathological properties
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DOI：
10.3892/ijmm.2015.2102
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发表时间：
2015-04-01
影响因子：
5.4
通讯作者：
Dong, Xiao-Ping
中科院分区：
文献类型：
--
作者：
Shi, Qi;Xiao, Kang;Dong, Xiao-Ping
朊病毒病是一种人畜共患疾病，可以通过自然传播和实验传播。在先前的研究中，我们证明了小鼠适应性羊瘙痒病菌株ME 7（ME 7-mo）和139 A（139 A-mo）在接种到金黄仓鼠中时可以克服种属障碍并诱导实验性羊瘙痒病，并且产生了2个新的仓鼠适应性菌株ME 7（ME 7-ha）和139 A（139 A-ha）。在本研究中，为了评估新形成的羊瘙痒病病原体的感染性和其他分子和神经病理学特性，将ME 7-ha和139 A-ha进一步脑内接种到仓鼠中。与第一代毒株感染相比，第二代毒株感染的潜伏期和临床病程明显缩短，与感染其亲代小鼠毒株的小鼠相当。在感染第2代这2种毒株的动物中，脑PrPSc的糖基化模式与感染第1代这些毒株的动物中的糖基化模式保持相似的特征，主要是二糖基化的PrPSc。神经病理学分析显示，在感染小鼠和仓鼠的脑组织中，海绵状变性和小胶质细胞增殖相当，但在仓鼠的大脑中，PrPSc的斑块样沉积物明显更多，星形胶质细胞增生更严重。这些数据表明，种间感染产生的菌株ME 7-ha 1st和139 A-ha 1st可以在新宿主仓鼠中传代，并稳定地保持其分子和神经病理学特征。
As a type of zoonotic disease, prion diseases may be transmited naturally and experimentally among species. In a previous study, we demonstrated that the mouse-adapted scrapie strains, ME7 (ME7-mo) and 139A (139A-mo), can overcome the species barrier and induce experimental scrapie when inoculated into Golden hamsters and generated 2 new hamster-adapted strains, ME7 (ME7-ha) and 139A (139A-ha). In the present study, in order to assess the infectivity and other molecular and neuropathological properties of the newly formed scrapie agents, ME7-ha and 139A-ha were further intracerebrally inoculated into hamsters. Compared with infection with 1st passage strains, the incubation times and clinical courses of infection with 2nd passage strains were markedly shorter, which were quite comparable with those of the mice infected with their parent mouse strains. The glycosylation patterns of brain PrPSc in the animals infected with the 2nd passage of those 2 strains maintained similar features as those in the animals infected with the 1st passage of those strains, with predominantly diglycosylated PrPSc. Neuropathological assays revealed comparable spongiform degeneration and microglia proliferation in the brain tissues from the infected mice and hamsters, but markedly more plaque-like deposits of PrPSc and more severe astrogliosis in the brains of the hamster. These data indicate that the strains, ME7-ha 1st and 139A-ha 1st generated by interspecies infection can passage in the new host hamster and stably maintain their molecular and neuropathological characteristics.