THE TTG GENE IS REQUIRED TO SPECIFY EPIDERMAL-CELL FATE AND CELL PATTERNING IN THE ARABIDOPSIS ROOT
THE TTG GENE IS REQUIRED TO SPECIFY EPIDERMAL-CELL FATE AND CELL PATTERNING IN THE ARABIDOPSIS ROOT
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DOI：
10.1006/dbio.1994.1352
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发表时间：
1994-12-01
影响因子：
2.7
通讯作者：
SCHIEFELBEIN, JW
中科院分区：
文献类型：
--
作者：
GALWAY, ME;MASUCCI, JD;SCHIEFELBEIN, JW
在拟南芥根表皮中研究了细胞命运的控制。通常存在两种不同类型的分化的表皮细胞：根毛细胞和无毛细胞。在野生型拟南芥根，表皮细胞的命运被发现与细胞的位置，根毛细胞位于径向壁之间的皮层细胞，和无毛细胞直接位于皮层细胞。这种正常的位置关系是不存在的TTG（透明种皮无毛）突变体（缺乏毛状体，花青素，种皮粘液），表皮细胞在所有位置分化成根毛细胞。相反的条件下产生的根的转基因拟南芥表达玉米R（R-Lc）基因产物（一个假定的TTG同源物）的控制下的强启动子（CaMV 35 S），产生无毛表皮细胞在所有位置。在TTG和R-表达根，表皮细胞分化的影响在早期阶段，细胞伸长或根毛形成的发病之前。ttg突变也与根尖分生组织内和周围细胞的形态和组织异常有关。结果表明，TTG活性的改变导致发育中的表皮细胞误解其位置并分化成不适当的细胞类型。这表明，在野生型根，TTG提供，或响应，位置信号，导致分化的表皮细胞，皮层细胞上的谎言，采取无毛细胞的命运。(C)1991年出版社出版。
The control of cell fate was investigated in the root epidermis of Arabidopsis thaliana. Two distinct types of differentiated epidermal cells are normally present: root-hair-bearing cells and hairless cells. In wild-type Arabidopsis roots, epidermal cell fate was found to be correlated with cell position, with root-hair cells located over radial walls between cortical cells, and with hairless cells located directly over cortical cells. This normal positional relationship was absent in ttg(transparent testa glabrous) mutants (lacking trichomes, anthocyanins, and seed coat mucilage); epidermal cells in all positions differentiate into root-hair cells. The opposite condition was generated in roots of transgenic Arabidopsis expressing the maize R (R-Lc) gene product (a putative TTG homologue) under the control of a strong promoter (CaMV35S), which produced hairless epidermal cells in all positions. In both the ttg and R-expressing roots, epidermal cell differentiation was affected at an early stage, prior to the onset of cell elongation or root-hair formation. The ttg mutations were also associated with abnormalities in the morphology and organization of cells within and surrounding the root apical meristem. The results indicate that alterations in TTG activity cause developing epidermal cells to misinterpret their position and differentiate into inappropriate cell types. This suggests that, in wild-type roots, TTG provides, or responds to, positional signals to cause differentiating epidermal cells that lie over cortical cells to adopt a hairless cell fate. (C) 1991 Academic Press, Inc.