Cell cycle markers have different expression and localization patterns in neuron-like PC12 cells and primary hippocampal neurons
Cell cycle markers have different expression and localization patterns in neuron-like PC12 cells and primary hippocampal neurons
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DOI：
10.1016/j.neulet.2011.03.100
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发表时间：
2011-06-01
影响因子：
2.5
通讯作者：
Karabay, Arzu
中科院分区：
文献类型：
--
作者：
Negis, Yesim;Unal, Aysegul Yildiz;Karabay, Arzu
在已发表的研究中，神经元样PC 12细胞被广泛用于代替神经元。本文的目的是比较mRNA和蛋白质表达的细胞周期标志物; cyclinA，B，D，E; Cdk 1，2和4;和p27在有丝分裂后的原代海马神经元，有丝分裂活跃的PC 12细胞和NGF分化的有丝分裂后的PC 12细胞。与PC 12细胞相反，在神经元中，所有这些标记物的存在仅在mRNA水平上检测到;除了细胞周期蛋白A、细胞周期蛋白E和Cdk 4，其在蛋白水平上也是可检测的。在神经生长因子处理的PC 12细胞和神经元中，cyclinE仅定位于细胞核。在神经生长因子处理的PC 12细胞中，cyclinD和Cdk 4定位于细胞核，而在神经元中，cyclinD的表达是不可检测的，Cdk 4定位于细胞质。在神经元中，cyclinA是核，而在NGF处理的PC 12细胞中，它定位于细胞体和沿着突起。这些结果表明，PC 12细胞和原代神经元的细胞周期蛋白的表达和定位是不同的。因此，使用这些细胞作为神经元模型系统来理解神经元生理活动可能不是非常合适的，上游可能存在细胞周期激活触发事件。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Neuron-like PC12 cells are extensively used in place of neurons in published studies. Aim of this paper has been to compare mRNA and protein expressions of cell cycle markers; cyclinA, B, D, E; Cdk1, 2 and 4; and p27 in post-mitotic primary hippocampal neurons, mitotically active PC12 cells and NGF-differentiated post-mitotic PC12 cells. Contrary to PC12 cells, in neurons, the presence of all these markers was detected only at mRNA level; except for cyclinA, cyclinE and Cdk4, which were detectable also at protein levels. In both NGF-treated PC12 cells and neurons, cyclinE was localized only in the nucleus. In NGF-treated PC12 cells cyclinD and Cdk4 were localized in the nucleus while, in neurons cyclinD expression was not detectable; Cdk4 was localized in the cytoplasm. In neurons, cyclinA was nuclear, whereas in NGF-treated PC12 cells, it was localized in the cell body and along the processes. These results suggest that PC12 cells and primary neurons are different in terms of cell cycle protein expressions and localizations. Thus, it may not be very appropriate to use these cells as neuronal model system in order to understand neuronal physiological activities, upstream of where may lie cell cycle activation triggered events. (C) 2011 Elsevier Ireland Ltd. All rights reserved.