ESTABLISHMENT AND CHARACTERIZATION OF HUMAN PANCREATIC-CANCER CELL-LINES IN TISSUE-CULTURE AND IN NUDE-MICE
ESTABLISHMENT AND CHARACTERIZATION OF HUMAN PANCREATIC-CANCER CELL-LINES IN TISSUE-CULTURE AND IN NUDE-MICE
复制标题
DOI：
10.1111/j.1349-7006.1990.tb03336.x
复制
发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
KONO, A
中科院分区：
文献类型：
--
作者：
IKEDA, Y;EZAKI, M;KONO, A
在组织培养和裸鼠体内建立了三种人胰腺癌细胞系，命名为KP-1 N、KP-2和KP-3。KP-1 N和KP-3肿瘤获自胰腺肿瘤的肝转移，KP-2肿瘤为原发性胰腺来源。KP-1 N和KP-2来源的患者肿瘤显示腺癌特征，KP-3肿瘤具有腺鳞癌特征。将来自手术标本的样品接种到无胸腺裸鼠中导致肿瘤形成，肿瘤在组织学上与原始肿瘤非常相似。皮下注射已建立的细胞系也诱导肿瘤形成，在KP-2和KP-3肿瘤的情况下，肿瘤在组织学上类似于原始病变，但小鼠中的KP-1 N肿瘤在组织学上与手术标本不同。KP-1 N、KP-2和KP-3细胞系已分别在补充有10%胎牛血清的培养基中连续培养超过20、21和17个月。KP-2和KP-3细胞系产生并释放碳水化合物抗原19-9到用过的培养基中，但KP-1 N细胞系没有。KP-1 N和KP-3在脾内注射到裸鼠中后产生肝转移，而KP-2产生很少的肝集落。分别从KP-1 N和KP-3的肝集落中分离高度转移至肝的细胞系KP-1 NL和KP-3Ls。
Three human pancreactic cancer cell lines, designated as KP-1N, KP-2 and KP-3 have been established in both tissue cultures and in nude mice. The KP-1N and KP-3 tumors were obtained from liver metastases of pancreatic tumors and the KP-2 tumor was of primary pancreatic origin. The patients'' tumors from which KP-1N and KP-2 were derived showed characteristics of adenocarcinoma, and the KP-3 tumor had adenosquamous carcinoma characteristics. Inoculations of samples from surgical specimens into athymic nude mice resulted in tumor formation, with the tumors histologically closely resembling the original neoplasms. Subcutaneous injections of the established cell lines also induced tumor formation and the tumors histologically resembled the original lesion in the cases of KP-2 and KP-3 tumors, but the KP-1N tumors in the mice were histologically different from the surgical specimen. The KP-1N, KP-2 and KP-3 cell lines have been cultured continuously in a medium supplemented with 10% fetal calf serum for more than 20, 21 and 17 months, respectively. The KP-2 and KP-3 cell lines produced and released carbohydrate antigen 19-9 into the spent medium but the KP-1N cell line did not. KP-1N and KP-3, produced liver metastases after intrasplenic injection into nude mice, whereas KP-2 produced few liver colonies. Cell lines highly metastatic to the liver, KP-1NLs and KP-3Ls, were isolated from the liver colonies of KP-1N and KP-3, respectively.