PKCδ is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements
PKCδ is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements
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DOI：
10.1101/gad.1101303
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发表时间：
2003-07-01
影响因子：
10.5
通讯作者：
Ueno, N
中科院分区：
文献类型：
--
作者：
Kinoshita, N;Iioka, H;Ueno, N
蛋白激酶C （PKC）与Wnt信号通路有关；然而，人们对其分子作用知之甚少。我们在爪蟾EST数据库中发现了新的编码delta型PKC的基因。PKCdelta功能的缺失揭示了它在原肠形成过程中的收敛延伸是必不可少的。然后我们研究了PKCdelta和Wnt通路之间的关系。PKCdelta响应卷曲信号转移到质膜上。此外，PKCdelta功能的缺失抑制了disheveled的易位和Frizzled对c-Jun n -末端激酶（INK）的激活。此外，PKCdelta与disheveled形成络合物，phobol酯激活PKCdelta足以实现disheveled易位和INK激活。因此，PKCdelta通过调节disheveled的定位和活性，在Wnt/JNK通路中发挥重要作用。
Protein kinase C (PKC) has been implicated in the Wnt signaling pathway; however, its molecular role is poorly understood. We identified novel genes encoding delta-type PKC in the Xenopus EST databases. Loss of PKCdelta function revealed that it was essential for convergent extension during gastrulation. We then examined the relationship between PKCdelta and the Wnt pathway. PKCdelta was translocated to the plasma membrane in response to Frizzled signaling. In addition, loss of PKCdelta function inhibited the translocation of Dishevelled and the activation of c-Jun N-terminal kinase (INK) by Frizzled. Furthermore, PKCdelta formed a complex with Dishevelled, and the activation of PKCdelta by phorbol ester was sufficient for Dishevelled translocation and INK activation. Thus, PKCdelta plays an essential role in the Wnt/JNK pathway by regulating the localization and activity of Dishevelled.