Nano-enabled sensing approaches for pathogenic bacterial detection
Nano-enabled sensing approaches for pathogenic bacterial detection
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DOI：
10.1016/j.bios.2020.112276
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发表时间：
2020-10-01
影响因子：
12.6
通讯作者：
Pan, Dipanjan
中科院分区：
文献类型：
--
作者：
Alafeef, Maha;Moitra, Parikshit;Pan, Dipanjan
由病原菌特别是耐药菌引起的传染病是全球健康面临的最大威胁之一。迄今为止，细菌污染是使用传统的培养技术检测的，这高度依赖于专家用户，受到处理时间和现场可用性的限制。因此，需要实时和连续监测病原体水平，以获得有价值的信息，这些信息可以帮助卫生机构指导预防和遏制病原体相关的疫情。基于纳米技术的智能传感器正在为在患者的护理点早期和快速检测此类病原体开辟新的途径。纳米材料由于其独特的光学、磁性和电学性质，在细菌传感中发挥着重要作用。碳纳米颗粒，金属纳米颗粒，金属氧化物纳米颗粒和各种类型的纳米复合材料是智能纳米材料的例子，在微生物检测领域引起了广泛的关注。这些方法，加上现代技术的出现，再加上机器学习和无线通信，代表了传染病诊断的未来趋势。本文综述了近年来成功利用不同纳米颗粒进行细菌检测的进展。首先，我们介绍了基于纳米颗粒的诊断平台的设计和策略背后的基本概念和机制。代表性的研究工作突出了在体外和体内检测细菌。一个全面的讨论，然后提出，以涵盖最常用的细菌鉴定技术，包括一些开创性的研究，以检测细菌在单细胞水平。最后，我们讨论了当前的挑战和该领域的前景，以及建议的解决方案。
Infectious diseases caused by pathogenic bacteria, especially antibiotic-resistant bacteria, are one of the biggest threats to global health. To date, bacterial contamination is detected using conventional culturing techniques, which are highly dependent on expert users, limited by the processing time and on-site availability. Hence, realtime and continuous monitoring of pathogen levels is required to obtain valuable information that could assist health agencies in guiding prevention and containment of pathogen-related outbreaks. Nanotechnology-based smart sensors are opening new avenues for early and rapid detection of such pathogens at the patient's point of-care. Nanomaterials can play an essential role in bacterial sensing owing to their unique optical, magnetic, and electrical properties. Carbon nanoparticles, metallic nanoparticles, metal oxide nanoparticles, and various types of nanocomposites are examples of smart nanomaterials that have drawn intense attention in the field of microbial detection. These approaches, together with the advent of modern technologies and coupled with machine learning and wireless communication, represent the future trend in the diagnosis of infectious diseases. This review provides an overview of the recent advancements in the successful harnessing of different nano particles for bacterial detection. In the beginning, we have introduced the fundamental concepts and mechanisms behind the design and strategies of the nanoparticles-based diagnostic platform. Representative research efforts are highlighted for in vitro and in vivo detection of bacteria. A comprehensive discussion is then presented to cover the most commonly adopted techniques for bacterial identification, including some seminal studies to detect bacteria at the single-cell level. Finally, we discuss the current challenges and a prospective outlook on the field, together with the recommended solutions.