Bacteriopheophytin g: Properties and some speculations on a possible primary role for bacteriochlorophylls b and g in the biosynthesis of chlorophylls.
Bacteriopheophytin g: Properties and some speculations on a possible primary role for bacteriochlorophylls b and g in the biosynthesis of chlorophylls.
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细菌脱镁叶绿素 g：细菌叶绿素 b 和 g 在叶绿素生物合成中可能的主要作用的特性和一些推测。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
J. Katz
中科院分区：
文献类型：
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作者：
T. J. Michalski;J. E. Hunt;M. Bowman;U. Smith;K. Bardeen;H. Gest;J. Norris;J. Katz
从最近发现的光合细菌chlorum Heliobacterium中获得了高纯度的bacteriopophytin g和少量细菌叶绿素g。描述了这些敏感化合物的制备方法和处理注意事项。这些化合物通过加利福尼亚-252等离子解吸质谱、高效液相色谱、可见吸收和电子自旋共振光谱进行了表征。我们的结果与H. Brockmann和A. Lipinski提出的细菌叶绿素g的结构一致[(1983)Arch。微生物，136,17-19]，除了我们发现酯化醇是法尼醇而不是最初建议的香叶醇。报道了三态细菌叶绿素g的零场分裂参数和细菌叶绿素g阳离子自由基的ESR特性。研究了该化合物的光异构化反应。在白光下，植菌素g与植菌素a进行光异构化，半衰期约为42分钟。我们认为所有的叶绿素都是由一种含有乙基的常见中间体[unk]CH-CH(3)合成的，就像存在于细菌叶绿素b和g中的叶绿素一样。
Bacteriopheophytin g and small amounts of bacteriochlorophyll g have been obtained in high purity from the recently discovered photosynthetic bacterium Heliobacterium chlorum. Preparative methods and precautions in handling these sensitive compounds are described. The compounds have been characterized by californium-252 plasma desorption mass spectrometry, HPLC, visible absorption, and electron spin resonance spectroscopy. Our results agree with the structure of bacteriochlorophyll g advanced by H. Brockmann and A. Lipinski [(1983) Arch. Microbiol. 136, 17-19], with the exception that we find the esterifying alcohol to be farnesol and not geranylgeraniol as originally suggested. Zero field splitting parameters of triplet state bacteriopheophytin g and the ESR properties of the cation free radical of bacteriochlorophyll g are reported. The photoisomerization of the subject compounds has been studied. Bacteriopheophytin g undergoes photo-isomerization in white light to pheophytin a with a half-time of approximately 42 min. We suggest that all of the chlorophylls are biosynthesized from a common intermediate containing an ethylidine group, [unk]CH-CH(3), such as is present in bacteriochlorophylls b and g.