C-phycocyanin extraction assisted by pulsed electric field from Artrosphira platensis
C-phycocyanin extraction assisted by pulsed electric field from Artrosphira platensis
复制标题
DOI：
10.1016/j.foodres.2016.09.029
复制
发表时间：
2017-09-01
影响因子：
8.1
通讯作者：
Raso, Javier
中科院分区：
文献类型：
--
作者：
Manuel Martinez, Juan;Luengo, Elisa;Raso, Javier
为了提高藻蓝蛋白在水介质中的提取效率，研究了脉冲电场(PEF)对钝顶弧菌新鲜生物量的影响。钝顶弧菌的电穿孔与电场强度和处理时间有关。提取液中C-藻蓝蛋白检测的最小电场强度为15kV/cm，处理时间为150ps(50个脉冲/3ps)。然而，较短的处理时间需要较高的电场强度。采用响应曲面法考察了电场强度(15~25kV/cm)、处理时间(60~150亩S)和脉冲电场处理温度(10~40℃)对藻蓝蛋白萃取率的影响。PEF处理温度的升高降低了获得给定PEY所需的电场强度和处理时间，从而降低了处理所提供的总比能。例如，温度从10℃升高到40℃，可以将提取100 mg/g d(W)C-藻蓝蛋白所需的电场强度从25千伏/厘米降低到18千伏/厘米，比能量输入从106.7 kJ/Kg降低到67.5kJ/Kg。从电穿孔细胞中获得的C-藻蓝蛋白提取液的纯度高于在细胞完全破坏的基础上用其他方法获得的纯度。(C)2016爱思唯尔有限公司。保留所有权利。
This paper assesses the application of pulsed electric fields (PEF) to the fresh biomass of Artrhospira platensis in order to enhance the extraction of C-phycocyanin into aqueous media.Electroporation of A. platensis depended on both electric field strength and treatment duration. The minimum electric field intensity for detecting C-phycocyanin in the extraction medium was 15 kV/cm after the application of a treatment time 150 ps (50 pulses of 3 ps). However higher electric field strength were required when shorter treatment times were applied. Response surface methodology was used in order to investigate the influence of electric field strength (15-25 kV/cm), treatment time (60-150 mu s), and temperature of application of PEF (10-40 degrees C) on C-phycocyanin extraction yield (PEY). The increment of the temperature PEF treatment reduced the electric field strength and the treatment time required to obtain a given PEY and, consequently decreased the total specific energy delivered by the treatment. For example, the increment of temperature from 10 degrees C to 40 degrees C permitted to reduce the electric field strength required to extract 100 mg/g d(w) of C-phycocyanin from 25 to 18 kV/cm, and the specific energy input from 106.7 to 67.5 kJ/Kg.Results obtained in this investigation demonstrated PEF's potential for selectively extraction C-phycocyanin from fresh A. platensis biomass. The purity of the C-phycocyanin extract obtained from the electroporated cells was higher than that obtained using other techniques based on the cell complete destruction. (C) 2016 Elsevier Ltd. All rights reserved.