Neuromedin U is involved in nociceptive reflexes and adaptation to environmental stimuli in mice.
Neuromedin U is involved in nociceptive reflexes and adaptation to environmental stimuli in mice.
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DOI：
10.1016/j.bbrc.2004.08.136
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发表时间：
2004-10
影响因子：
3.1
通讯作者：
K. Nakahara;M. Kojima;R. Hanada;Yutaka Egi;T. Ida;M. Miyazato;K. Kangawa;N. Murakami
中科院分区：
文献类型：
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作者：
K. Nakahara;M. Kojima;R. Hanada;Yutaka Egi;T. Ida;M. Miyazato;K. Kangawa;N. Murakami
在我们最近观察到NMU基因敲除(NMU KO)小鼠不活动和运动缓慢之后，我们比较了NMU KO和野生型小鼠的伤害性反射和环境适应。热板和福尔马林试验显示，NMU KO小鼠对热和痛的反射明显减少。相反，野生型小鼠脑室注射NMU以剂量依赖的方式刺激伤害性反射。注射NMU后，c-Fos在下丘脑、脑干和脊髓的广泛部位表达增加。福尔马林注射后2、4h，脊髓NMU基因表达增加，而下丘脑NMU基因表达无明显变化。当它们的明暗周期提前或延迟5小时时，NMU KO小鼠需要比野生型小鼠更长的时间重新进入新的明暗周期。野生型小鼠在环境温度从23°C改变到37°C后血压升高，而NMU KO小鼠则没有观察到这种升高。束缚应激10min后，野生型小鼠血皮质酮水平显著升高，而NMU KO小鼠则无明显变化。这些结果提示，内源性NMU可能参与了对环境刺激的反射和适应。
Following our recent observations of inactivity and slowed movement in neuromedin U knockout (NMU KO) mice, we compared nociceptive reflexes and environmental adaptation in NMU KO and wild-type mice. Hot plate and formalin tests revealed that reflexes to heat and pain were significantly decreased in NMU KO mice. Conversely, intracerebroventricular injection of NMU into wild-type mice stimulated nociceptive reflexes in a dose-dependent manner. After NMU injection, increased c-Fos expression was observed in a wide range of locations in hypothalamus, brainstem, and spinal cord. NMU mRNA expression increased in the spinal cord, but not in the hypothalamus, 2 and 4h after formalin injection. When their light–dark cycle was advanced or retarded by 5h, NMU KO mice required a longer time to re-entrain into the new light–dark cycle than did wild-type mice. Wild-type mice displayed increased blood pressure after their environmental temperature was changed from 23 to 37°C, whereas no such increase was observed in NMU KO mice. Blood corticosterone levels were significantly increased after 10min of immobilization stress in wild-type mice, but not in NMU KO mice. These results suggest that endogenous NMU may be involved in reflexes and adaptation to environmental stimuli.