Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
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DOI：
10.1021/acs.analchem.6b03536
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发表时间：
2017-01-17
影响因子：
7.4
通讯作者：
Choo, Jaebum
中科院分区：
文献类型：
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作者：
Wang, Xiaokun;Choi, Namhyun;Choo, Jaebum
研制了一种新型的基于表面增强拉曼散射（Sers）的侧向流动分析（LFA）生物传感器，用于同时检测双DNA标记物。该传感器中的LFA条由两条测试线和一条对照线组成。利用Sers纳米标签标记检测DNA探针，以高灵敏度对双DNA标记物进行定量评价。对卡波西肉瘤相关疱疹病毒（KSHV）和杆菌性血管瘤病（BA）相关的靶DNA进行了测试，以验证这种基于SERS的LFA条的检测能力。两条测试线上的Sers纳米标签的特征峰强度用于定量评估KSHV和BA。KSHV和BA的检测限，从我们的SERS为基础的LFA传感平台确定，估计分别为0.043和0.074 pM。这些值指示比先前使用基于聚集的比色法报道的值高约10000倍的灵敏度。我们相信，这是第一个报告的同时检测两种不同的DNA混合物使用SERS为基础的LFA平台。这种新的检测技术也是一个有前途的多重DNA传感平台，用于早期疾病诊断。
A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposis sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.