ESTABLISHMENT AND CHARACTERIZATION OF THE HUMAN MEDULLOBLASTOMA CELL-LINE AND TRANSPLANTABLE XENOGRAFT D283-MED
ESTABLISHMENT AND CHARACTERIZATION OF THE HUMAN MEDULLOBLASTOMA CELL-LINE AND TRANSPLANTABLE XENOGRAFT D283-MED
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DOI：
10.1097/00005072-198511000-00005
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
BIGNER, DD
中科院分区：
文献类型：
--
作者：
FRIEDMAN, HS;BURGER, PC;BIGNER, DD
一种新的连续细胞系和可移植的异种移植物，D283 Med，来源于一名转移性髓母细胞瘤儿童的腹膜植入物和腹水，并在体外悬浮培养中自发形成宏观球体。体外扩增时间为52.55 h。琼脂糖培养基中菌落形成的平均效率为1.83 +-。0.56%。D283 Med细胞系在胸腺小鼠体内生长，作为连续移植的颅内和皮下异种移植物。颅内肿瘤以小细胞团的形式生长，细胞质少，有丝分裂象多，有明显的核带，类似于神经母细胞的玫瑰花。皮下（SQ）肿瘤是明显的细胞肿瘤，但不包含莲座。它们表达谷氨酰胺合成酶、神经元特异性烯醇化酶和神经丝蛋白。未检测到胶质原纤维酸性蛋白和S-100蛋白。SQ肿瘤生长至500 mm3，潜伏期为52.55±±。12.5天，翻倍时间9.33 +-。2.39天。腹膜植入体和腹水细胞的茎系核型包含一个额外的11号染色体拷贝和三条标记染色体（8q+、17p+、20q+）。培养的细胞系和皮下、颅内异种移植物保留了3条标记染色体，与原始核型的不同之处在于它们缺少11号染色体的额外拷贝。该细胞系和可移植的异种移植物可以进一步分析人髓母细胞瘤的生物学特性和治疗敏感性。
A new continuous cell line and transplantable xenogrft, D283 Med, was derived from the peritoneal implant and ascitic fluid of a child with metastatic medulloblastoma and grew in vitro in suspension culture with spontaneous macroscopic spheroid formation. The in vitro population doubling time was 52.55 hours. Mean colony forming efficiency in an agarose medium was 1.83 .+-. 0.56%. The cell line, D283 Med, grew in athymic mice as serially transplantable intracranial and subcutaneous xenografts. Intracranial tumors grew as masses of small cells with scant cytoplasm and abundant mitotic figures and prominent anuclear zones resembling neuroblastic rosettes. Subcutaneous (SQ) tumors were markedly cellular neoplasms but did not contain rosettes. They expressed glutamine synthetase, neuron-specific enolase and neurofilament protein. Glial fibrillary acidic protein and S-100 protein were not detected. The SQ tumors grew to 500 mm3 with a latency of 52.55 .+-. 12.5 days and a doubling time of 9.33 .+-. 2.39 days. The stemline karyotypes of the peritoneal implant and ascitic fluid cells contained an extra copy of chromosome number 11 and three marker chromosomes (8q+, 17p+, 20q+). The cultured cell line and subcutaneous and intracranial xenografts retained the three marker chromosomes and differed from the original karyotype only in that they lacked the additional copy of chromosome number 11. This cell line and transplantable xenograft may allow further analysis of the biological properties and therapeutic sensitivty of human medulloblastoma.